Balloon Bacteria : Chiba 2006
From 2006.igem.org
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'''→World first bacteria that swells by lights.!!!''' | '''→World first bacteria that swells by lights.!!!''' | ||
| + | '''When growing...''' | ||
| - | =Design= | + | The movement of the E. coli can be obvered with the naked eyes ! |
| + | |||
| + | "Big" E. coli is easily sorted by FACS ! | ||
| + | |||
| + | Make much more proteins/cell ! | ||
| + | |||
| + | ==Design== | ||
'''First''' | '''First''' | ||
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The E. coli doesn't have photoreceptor. But... | The E. coli doesn't have photoreceptor. But... | ||
| - | '''We have Cph8( | + | '''We have Cph8(utwxas/uCSF;iGEM2005)in our hand''' |
| + | |||
| + | '''Second''' | ||
| + | |||
| + | The E. coli divides the cells by forming "partition". So... | ||
| + | |||
| + | ''''Expression of the enzyme in septem formation is knocked-down.''' | ||
| + | |||
| + | '''Third''' | ||
| + | |||
| + | E. coli rather "extend" due to the action of RodA. So... | ||
| + | |||
| + | '''We make inducible RodA''mut''. ''' | ||
| + | |||
| + | [[Image:design3.jpg]] | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | ==Experiments== | ||
| + | |||
| + | #We made inducible rodA''mut''. | ||
| + | #We made CI inverter and compared it. | ||
| + | |||
| + | |||
| + | |||
| + | ==Results== | ||
| + | |||
| + | '''1.rodA''mut'' ''' | ||
| + | |||
| + | [[Image:rodAmut2.jpg]] | ||
| + | |||
| + | ...show more.[[rodAmut]] | ||
| + | |||
| + | '''2.CI inverter''' | ||
| + | [[Image:CIinverter3.jpg]] | ||
| + | ...show more.[[CI inverter]] | ||
| - | == Member == | + | ==Member== |
[[Chiba University, Japan 2006|Chiba 2006]] | [[Chiba University, Japan 2006|Chiba 2006]] | ||
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'''『je』team''' | '''『je』team''' | ||
*[[User:Asari|Yuki Asari]] | *[[User:Asari|Yuki Asari]] | ||
| - | |||
*[[User:Kaneda|Tomoko Kaneda]] | *[[User:Kaneda|Tomoko Kaneda]] | ||
*[[User:Megumi|Ken Megumi]] | *[[User:Megumi|Ken Megumi]] | ||
*[[User:Yasuno|Kayo Yasuno]] | *[[User:Yasuno|Kayo Yasuno]] | ||
Latest revision as of 11:37, 30 October 2006
Contents |
Concept
The light in the morning leaking from a gap between the curtains... this morning lights calls and awakes the E. coli.
Suprise!!
The E. coli become bigger and bigger like BALLOON!!!
→World first bacteria that swells by lights.!!!
When growing...
The movement of the E. coli can be obvered with the naked eyes !
"Big" E. coli is easily sorted by FACS !
Make much more proteins/cell !
Design
First
The E. coli doesn't have photoreceptor. But...
We have Cph8(utwxas/uCSF;iGEM2005)in our hand
Second
The E. coli divides the cells by forming "partition". So...
'Expression of the enzyme in septem formation is knocked-down.
Third
E. coli rather "extend" due to the action of RodA. So...
We make inducible RodAmut.
Experiments
- We made inducible rodAmut.
- We made CI inverter and compared it.
Results
1.rodAmut
...show more.rodAmut
2.CI inverter
...show more.CI inverter
Member
Chiba 2006 http://chem.tf.chiba-u.jp/igem/ iGEM Chiba
『je』team
