Presentation

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The Powerpoint can be found here: [[Media:Blue.Water.Presentation.ppt]]
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The Powerpoint can be found here:  
 +
[http://www.individual.utoronto.ca/chuck_1985/UT.Waterloo.iGEM.2006.Presentation.ppt Presentation]
 +
 
 +
[[University_of_Toronto_2006]]
== Presentation Script ==
== Presentation Script ==
-
Script:
+
====Slide 1====
-
 
+
* Introduce the team (i.e. collaborative effort between UofT and UW) and mention the project we're working on.
-
Slide 1:
+
-
- Introduce the team (i.e. collaborative effort between UofT and UW) and mention the project we're working on.
+
-
 
+
-
Slide 2:
+
-
- Explain what makes this thermometer unique from all other existing models as in other models may limitations to where/how they can be used
+
-
- size of the species of interest may be too small
+
-
- research experiments where chemicals, radiation may be toxic for a colony but temperature readings are needed
+
-
 
+
-
- A bio-synthetic thermometer may be a possible solution to overcome these obstacles.  
+
-
- Applications:
+
====Slide 2====
-
- use to detect small changes in temperature in very small organisms- we can do this by measuring the intensity of the colour emitted
+
* Explain what makes this thermometer unique from all other existing models as in other models may limitations to where/how they can be used
-
- experiments or places where a quantitative reading cannot be obtained (i.e a person can not physically be there to take the reading (in anaerobic conditions?)) or where air pressure
+
** size of the species of interest may be too small
-
- may also use this to monitor temperature of aquariums using zebra fish for example
+
** research experiments where chemicals, radiation may be toxic for a colony but temperature readings are needed
-
Slide 3:
+
* A bio-synthetic thermometer may be a possible solution to overcome these obstacles.
-
- Explain diagrams
+
-
Slide 4:
+
* Applications:
-
- Explain our approach: how we decided to ligate parts separately and build up to the final product
+
** use to detect small changes in temperature in very small organisms- we can do this by measuring the intensity of the colour emitted
-
- Constructed many new parts
+
** experiments or places where a quantitative reading cannot be obtained (i.e a person can not physically be there to take the reading (in anaerobic conditions?)) or where air pressure
-
- Replaced our cI repressor system with tetracycline repressor (tetR), an analogous system to cI but less leaky and can control externally
+
** may also use this to monitor temperature of aquariums using zebra fish for example
-
Slide 5:
+
====Slide 3====
-
- Testing Phase
+
* Explain diagrams
-
- Intro: - Testing was split into 3 modules.
+
-
- Each module acts as a checkpoint for a particular milestone in the construction process.
+
-
- After each module we can ensure a vital component of the construct is functional.
+
-
- State the goal/purpose of each module and how it was executed.
+
-
Slide 6:
+
====Slide 4====
-
- Test Results
+
* Explain our approach: how we decided to ligate parts separately and build up to the final product
 +
* Constructed many new parts
 +
* Replaced our cI repressor system with tetracycline repressor (tetR), an analogous system to cI but less leaky and can control externally
-
- Tested the intermediary pBad/araC + LacI ts coding sequence (R0011) + reporter gene thoroughly
+
====Slide 5====
-
- We ensured that module 1 tests passed before continuing construction.
+
* Testing Phase
-
+
** Intro: - Testing was split into 3 modules.
 +
*** Each module acts as a checkpoint for a particular milestone in the construction process.
 +
*** After each module we can ensure a vital component of the construct is functional.
 +
** State the goal/purpose of each module and how it was executed.
-
Slide 7
+
====Slide 6====
-
Conclusions/Results!
+
* Test Results
-
- Does it work/or not?
+
** Tested the intermediary pBad/araC + LacI ts coding sequence (R0011) + reporter gene thoroughly
-
- What we learned from constructing the device
+
** We ensured that module 1 tests passed before continuing construction.
-
- at the beginning, we tested all enzymes for functionality via test plasmid + running a gel - while doing this we learned that Xba1 works slower than EcoRI, SpeI, and PstI
+
-
- Team communication, allocate more time for testing in addition to construction/designate a test team
+
-
- Limitations to the device
+
-
- can only detect temperatures in a small range
+
-
- does fluorescent protein stay? or decay quickly? ask Charles/Andy
+
-
- Experiments involving radiation - this thermometer cannot be used as the radiation will damage the DNA
+
-
- Expression of the reporter gene must be as sensitive as the activation of the transcription factor (for accuracy in timing events dependent on temperature and time)
+
-
- Future work that can be done to improve the device/design for next year
+
-
- investigate repressors etc that would be good for larger temperature ranges
+
-
- this would increase the application of the device allowing it to be another alternative to detecting temperature changes in biological systems.  
+
 +
====Slide 7====
 +
* Conclusions/Results!
 +
** Does it work/or not?
 +
** What we learned from constructing the device
 +
*** at the beginning, we tested all enzymes for functionality via test plasmid + running a gel - while doing this we learned that Xba1 works slower than EcoRI, SpeI, and PstI
 +
*** Team communication, allocate more time for testing in addition to construction/designate a test team
 +
** Limitations to the device
 +
*** can only detect temperatures in a small range
 +
*** does fluorescent protein stay? or decay quickly? ask Charles/Andy
 +
*** Experiments involving radiation - this thermometer cannot be used as the radiation will damage the DNA
 +
*** Expression of the reporter gene must be as sensitive as the activation of the transcription factor (for accuracy in timing events dependent on temperature and time)
 +
*** Future work that can be done to improve the device/design for next year
 +
*** investigate repressors etc that would be good for larger temperature ranges
 +
*** this would increase the application of the device allowing it to be another alternative to detecting temperature changes in biological systems.
-
Slide 8:
+
====Slide 8====
-
- Team Members
+
* Team Members
-
Slide 9:
+
====Slide 9====
-
- Acknowledgements
+
* Acknowledgements

Latest revision as of 02:07, 8 December 2006

The Powerpoint can be found here: [http://www.individual.utoronto.ca/chuck_1985/UT.Waterloo.iGEM.2006.Presentation.ppt Presentation]

University_of_Toronto_2006

Contents

Presentation Script

Slide 1

  • Introduce the team (i.e. collaborative effort between UofT and UW) and mention the project we're working on.

Slide 2

  • Explain what makes this thermometer unique from all other existing models as in other models may limitations to where/how they can be used
    • size of the species of interest may be too small
    • research experiments where chemicals, radiation may be toxic for a colony but temperature readings are needed
  • A bio-synthetic thermometer may be a possible solution to overcome these obstacles.
  • Applications:
    • use to detect small changes in temperature in very small organisms- we can do this by measuring the intensity of the colour emitted
    • experiments or places where a quantitative reading cannot be obtained (i.e a person can not physically be there to take the reading (in anaerobic conditions?)) or where air pressure
    • may also use this to monitor temperature of aquariums using zebra fish for example

Slide 3

  • Explain diagrams

Slide 4

  • Explain our approach: how we decided to ligate parts separately and build up to the final product
  • Constructed many new parts
  • Replaced our cI repressor system with tetracycline repressor (tetR), an analogous system to cI but less leaky and can control externally

Slide 5

  • Testing Phase
    • Intro: - Testing was split into 3 modules.
      • Each module acts as a checkpoint for a particular milestone in the construction process.
      • After each module we can ensure a vital component of the construct is functional.
    • State the goal/purpose of each module and how it was executed.

Slide 6

  • Test Results
    • Tested the intermediary pBad/araC + LacI ts coding sequence (R0011) + reporter gene thoroughly
    • We ensured that module 1 tests passed before continuing construction.

Slide 7

  • Conclusions/Results!
    • Does it work/or not?
    • What we learned from constructing the device
      • at the beginning, we tested all enzymes for functionality via test plasmid + running a gel - while doing this we learned that Xba1 works slower than EcoRI, SpeI, and PstI
      • Team communication, allocate more time for testing in addition to construction/designate a test team
    • Limitations to the device
      • can only detect temperatures in a small range
      • does fluorescent protein stay? or decay quickly? ask Charles/Andy
      • Experiments involving radiation - this thermometer cannot be used as the radiation will damage the DNA
      • Expression of the reporter gene must be as sensitive as the activation of the transcription factor (for accuracy in timing events dependent on temperature and time)
      • Future work that can be done to improve the device/design for next year
      • investigate repressors etc that would be good for larger temperature ranges
      • this would increase the application of the device allowing it to be another alternative to detecting temperature changes in biological systems.

Slide 8

  • Team Members

Slide 9

  • Acknowledgements
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