Prairie View A&M University 2006

From 2006.igem.org

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-	== Project==	+	== Students ==
		+	[[Image:IGEM-Team2k6-sml.jpg ]]
		+	----
		+	[[More Group Pictures]]
		+	== Advisors ==
		+	'''Dr Raul Cuero,(PhD)'''
-	== Project Planning==	+	[[Image:DRcuero.jpg]]
-	== Students ==	+	Dr. Raul Cuero, (PhD), Microbiologist, Distinguished Professor.Research Leader, Scientist and Contact Person for Prairie View A&M University iGEM 2006 group.
		+	Dr Cuero holds 8 scientific inventions and more than 90 scientific publications.Dr Cuero is currently working on fungal toxins at the molecular level, and on biogenesis. He is also the author of the book "Triumph and Survival".
-	PVGroup.jpg [[Image:GroupPV.jpg]]	+	Email: olimpa@aol.com
-	Laina P. Magaya	+	www.raulcuerobiotech.com
-	Leslie C.Caulfield
-	Christian Cruz
-	Osayamen Edigin	+	'''Dr Harriet Howard-Lee Block'''
		+
		+	[[Image:Lee.jpg]]
-	Aneka Atkinson	+	[[Dr Block]]
-	== Advisors ==	+
-	Raul Cuero, PhD	+	'''Dr Dierdre Vaden'''
-	[[Image:DRcuero.jpg]]	+	[[Image:DrVaden.jpg]]
		+	[[Dr Vaden]]
		+
		+
		+	'''Dr Huajan Fan'''
-	Dr. William	+	[[Image:DrFan.jpg]]
		+
		+	[[Dr Fan]]
	== Student Profiles ==		== Student Profiles ==
-		+
		+	[[Laina]] | [[Leslie]] | [[Christian]] | [[George]] | [[Oludayo]] | [[Jessica]] | [[Terry]]|[[Fredrick]] | [[Victor]]
		+
		+	Face File [[https://static.igem.org/mediawiki/2006/2/20/Pictures.ppt]]
-	Laina	+	== Project ==
-	[[Image:Laina.jpg]]
-	My name is Laina P. Magaya and a Biology Major at Prairie View A&M University. Currently I am working on in vitro, environmental microbial bioremiodation of metal ions and radionuclides at Prairie View A&M University under the Principal Inevstigator Dr R. Cuero. I have also worked on microbiology techniquies involved in studies of ''Helicobatcer pylori'' and using plasmids from ''E.coli''.	+	(We have changed the title of our project so many times)
-	Leslie	+	'''Tri-Metallic Biosensor'''- PVAMU [[https://static.igem.org/mediawiki/2006/b/b9/Slide_Show2a.ppt]]
-	[[Image:Leslie Pic.jpg]]	+	'''Background'''
		+
		+	There exists two essential needs which enabled us to design the trimetallic probe. The need for alternative forms of oil, in fact their methods and the bioremediation of metals ions from the environment.
-	My name is Lesli Caulfield and I am a chemical engineering senior at Prairie View A&M University in Prairie View, TX.  I am honored to say that I have experience in what I want to accomplish.  While attending the University of Oklahoma, I was able to become a research assistant, for the summer of 2003, under Dr. David Schmidtke dealing with biosensors and cell adhesion.  Here, I worked with and constructed carbon nanotubes (regular and purified), made different types of PDMS mold and stamps, and just various types of lab work.  I personally, had fun discovering new things with the four other members of his research team. I had the opportunity to participate in the University of Kansas REU (CEBC) last summer under Dr. Aaron Scurto doing characterization of chiral sulfoxidation using HRP to expand my horizons in environmental engineering. Currently, I am working in a bio-fermentation lab (CEBC) under Dr. Michael Gyamerah with four of my peers.  I am studying the growth affects on a strand of E. Coli with different solvents.
-	As one can see, I have a diverse and unique research background.  I am interested in Dr. Cuero’s research because it will allow me to not only enhance my research opportunities a bit further, but it will allow the creative side of me to be nurtured and encouraged.  Being that he is one of the elite researchers’, I know I will learn a lot from.
		+	Micrococcus luteus strain (ATCC 4698) was transformed by plasmid pUC57-S-3M, in which Fe (II), Ni (II) and V (II) were fused to the fluorescence proteins (ECFP, EYFP, and mRFP respectively and to a fluorescent protein coding region [C0061(lux I)] ligated with a signaling sequence BBa_I13207.
-	Osayamen	+	Favorite Biobrick Map(Upcoming)
-	[[Image:osayamen1.jpg]]	+	The pUC57-S-3M was standardized for its specific sensoribility response to three metal ions and to sulfur (S), in order to detect metal contamination and/or hydrocarbons associated to these metals. Single and combination of the tree metals were used at different concentrations (0.2, 2, 50 ppm). The pUC57-S-3M was grown in the presence and absence of oxygen and/or hydrocarbon (Thiophenol). Only results from combined metals are reported. The biosensoribility was determined by the response of the pUC57-S-3M to the different concentrations of the metals. This response was measured by bioluminescence, fluorescence, DNA concentration, bacterial growth. These parameters were related to sensoribility of the pUC57-S-3M.
-	Aneka	+	'''Parts used'''
-	[[Image:Aneka_.jpg]]	+	<partinfo>BBa_S01984</partinfo>
		+	<partinfo>BBa_I13908</partinfo>/
		+	<partinfo>BBa_I13519</partinfo>/
		+	<partinfo>BBa_I4204</partinfo>/
		+	<partinfo>BBa_C0061</partinfo>/
		+	<partinfo>BBa_E0422</partinfo>/
		+	<partinfo>BBa_E0430</partinfo>/
		+	<partinfo>BBa_I13207</partinfo>/
		+	<partinfo>BBa_I13507</partinfo>/
-	Christian	+	'''Favorite Parts/ A promoter Biosenser <partinfo>BBa_J48108</partinfo>
-		+
-	[[Image:Chris2.jpg]]	+	<partinfo>BBa_J48108 specifiedcomponents</partinfo>
		+
		+	== Project Planning Diary ==
		+
		+	'''Calender'''
		+
		+	'''May'''
		+
		+	-Brainstorming and recruiting.
		+
		+	'''June'''
		+	No entry
		+
		+	'''July'''
		+
		+	'''August''
		+	'
		+	-We have been able to isolate a few colonies of transformants of E.coli containing the  plasmid 19H (pSB1A2 or BBa I13543). We have been bent on picking up sequences containig lux genes.
		+
		+	'''September'''
		+
		+	-So far we have tried to isolate several plasmids but results have not been good.  So far we have successfully amplified and isolated  4 plasmids. Well no one will believe how much we got done in such a small amout of time.  [Laina's Diary]
		+
		+	'''October'''
		+	Determined that we will base our sensor on the concentrations of three metals ions, Vanadium, Iron and Nickel,and including elemental sulfur. Our focus is on the DNA expression, determined by luminesence and flourescence.
		+
		+	== '''References''' ==
		+
		+	1.Cuero, R, Oullet  T. Metal ions modulate gene expression and accumulation of the mycotoxins aflatoxin and zearalenone.J Appl Microbiol. 2005;98(3):598-605. �
		+
		+	2.Lovley, DR (2003). "Cleaning up with genomics: applying molecular biology to bioremediation". NATURE REVIEWS. MICROBIOLOGY. 1 (1): 35–44. PMID 15040178.
		+	Holmes et al 1993b. Holmes D.S et al, 1993. Development of Biosensor to Measure Metal Ions
		+
		+	3. Bioavailability in mining and metal waste.pp659-666. In Biohydrometallurgical Technologies. Vol. II. A.E Torma, M.L Apel and C.L Brierle,TMS, Warrendale, PA,USA.Eds.
		+
		+	4.Holmes, D.S. Dubey, U.K and Gongolli, 1993. Development of Biosensor for the detection of Mercury andCopper Ions.Environ, Geochem. Health 16: 229-233.
-	My name is Christian Cruz, I am a graduate student of chemical engineering at Prairie View A&M university. I have been working with Dr. Raul Cuero for the last two years. At the beginning it was difficult for me because as engineer, I did know much about the biology work that was done at the laboratory, like bacteria growth and others. With time and the help of everyone at the laboratory now I am able to do different thing around like RNA, DNA extraction, things that I never thought I would do. Now with iGEM 2006 I see a chance for myself to put in practice all of my engineering knowledge.	+	5.Leonard G. Davis, Mark D. Dibner, James F Battey. Basic Methods in Molecular Biology. Elsevier Science Publishers.1986, New York, Amsterdam and London.

---

Latest revision as of 11:37, 4 November 2006

Contents 1 Students 2 Advisors 3 Student Profiles 4 Project 5 Project Planning Diary 6 References

Students

---

More Group Pictures

Advisors

Dr Raul Cuero,(PhD)

Dr. Raul Cuero, (PhD), Microbiologist, Distinguished Professor.Research Leader, Scientist and Contact Person for Prairie View A&M University iGEM 2006 group. Dr Cuero holds 8 scientific inventions and more than 90 scientific publications.Dr Cuero is currently working on fungal toxins at the molecular level, and on biogenesis. He is also the author of the book "Triumph and Survival".

Email: olimpa@aol.com

www.raulcuerobiotech.com

Dr Harriet Howard-Lee Block

Dr Block

Dr Dierdre Vaden

Dr Vaden

Dr Huajan Fan

Dr Fan

Student Profiles

Laina | Leslie | Christian | George | Oludayo | Jessica | Terry|Fredrick | Victor

Face File [[1]]

Project

(We have changed the title of our project so many times)

Tri-Metallic Biosensor- PVAMU [[2]]

Background

There exists two essential needs which enabled us to design the trimetallic probe. The need for alternative forms of oil, in fact their methods and the bioremediation of metals ions from the environment.

Micrococcus luteus strain (ATCC 4698) was transformed by plasmid pUC57-S-3M, in which Fe (II), Ni (II) and V (II) were fused to the fluorescence proteins (ECFP, EYFP, and mRFP respectively and to a fluorescent protein coding region [C0061(lux I)] ligated with a signaling sequence BBa_I13207.

Favorite Biobrick Map(Upcoming)

The pUC57-S-3M was standardized for its specific sensoribility response to three metal ions and to sulfur (S), in order to detect metal contamination and/or hydrocarbons associated to these metals. Single and combination of the tree metals were used at different concentrations (0.2, 2, 50 ppm). The pUC57-S-3M was grown in the presence and absence of oxygen and/or hydrocarbon (Thiophenol). Only results from combined metals are reported. The biosensoribility was determined by the response of the pUC57-S-3M to the different concentrations of the metals. This response was measured by bioluminescence, fluorescence, DNA concentration, bacterial growth. These parameters were related to sensoribility of the pUC57-S-3M.

Parts used

/ / / / / / / /

Favorite Parts/ A promoter Biosenser

Project Planning Diary

Calender

May

-Brainstorming and recruiting.

June No entry

July

'August ' -We have been able to isolate a few colonies of transformants of E.coli containing the plasmid 19H (pSB1A2 or BBa I13543). We have been bent on picking up sequences containig lux genes.

September

-So far we have tried to isolate several plasmids but results have not been good. So far we have successfully amplified and isolated 4 plasmids. Well no one will believe how much we got done in such a small amout of time. [Laina's Diary]

October Determined that we will base our sensor on the concentrations of three metals ions, Vanadium, Iron and Nickel,and including elemental sulfur. Our focus is on the DNA expression, determined by luminesence and flourescence.

References

1.Cuero, R, Oullet T. Metal ions modulate gene expression and accumulation of the mycotoxins aflatoxin and zearalenone.J Appl Microbiol. 2005;98(3):598-605. �

2.Lovley, DR (2003). "Cleaning up with genomics: applying molecular biology to bioremediation". NATURE REVIEWS. MICROBIOLOGY. 1 (1): 35–44. PMID 15040178. Holmes et al 1993b. Holmes D.S et al, 1993. Development of Biosensor to Measure Metal Ions

3. Bioavailability in mining and metal waste.pp659-666. In Biohydrometallurgical Technologies. Vol. II. A.E Torma, M.L Apel and C.L Brierle,TMS, Warrendale, PA,USA.Eds.

4.Holmes, D.S. Dubey, U.K and Gongolli, 1993. Development of Biosensor for the detection of Mercury andCopper Ions.Environ, Geochem. Health 16: 229-233.

5.Leonard G. Davis, Mark D. Dibner, James F Battey. Basic Methods in Molecular Biology. Elsevier Science Publishers.1986, New York, Amsterdam and London.