McGill University 2006

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=''Name: Fousion''=
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<center>[[Image:Banner.gif]]</center>
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= News =
 
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* '''2006-04-14''' Discussion page created
 
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* '''2006-03-16''' First kick-off meeting and team selection
 
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= Organisation =
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'''Welcome to the McGill Wiki!'''
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Basically, we're a team of 12 undergrads, 1 grad student, and 1 professor who like to have fun and clone things in our spare time. McGill University is located in Montreal, Quebec, which has given us wonderful opportunities to balance lab work with festivals and general craziness. iGEM has been a great opportunity to get lab experience while having the freedom to be creative, and we look forward to meeting the other teams at the jamboree!
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== People ==
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[[Image:Clip_image002.jpg]][[Image:Good_2.jpg]]
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=== Students ===
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|[[Catie Lichten (Center for Nonlinear Dynamics)]]
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|[[Octavio Mondragon (Physics)]]
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|[[Belinda Kong (Microbiology and Immunology)]]
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|[[Adrian Kaats (Biomedical Engineering)]]
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|[[Jamie Schafer (Microbiology and Immunology)]]
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|[[Horia Vulpe (Physiology)]]
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|[[Ashwini Bapat (Biochemistry)]]
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|[[Aaron Lapierre (Anatomy & Cell Biology)]]
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|[[Josh Wright (Chemistry)]]
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|[[Jieun Kim(Biology)]]
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|}
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=== Supervisors ===
 
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|[[Jay Nadeau]]
 
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|}
 
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== Timeline ==
 
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* [[Meetings]]
 
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== Tasks ==
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* [[Task Assignment]]
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EDITED BY ASHWIN. If you want to change it, feel free :)-->
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== Parts shopping list==
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<center><h2><font face="broadway,verdana">Projects</font></h2></center>
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Composite bricks - these are the best to use as less cloning is required.
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<table cellpadding="5" border="0"><tr>
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<h3><ul><li>'''Team 1: Split YFP'''</ul></h3>
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This project on fluorescence complementation involves the joining of two proteins, Jun and Fos, each fused to a half terminus of YFP. Both of these chimeric proteins were fused to a beta gene that codes for a membrane protein. Then, two cell populations - one expressing Jun-beta-YFPN and the other Fos-beta-YFPC - were combined, ideally resulting in the fusion of the Jun and Fos proteins on the cell membrane. The two halves of the YFP protein would bind as well, giving rise to fluorescence.
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'''All these bricks express YFP and downregulate it in response to a signal'''
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[[Background|Background]]
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1. Arabinose response
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[[Methods and Materials|Methods and Materials]]
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BBa_E0610: Arabinose turns on lac repressor which in turn shuts down Yellow fluorescent protein.
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[[Results|Results]]
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BBa_E0600: like BBa_E0610 but YFP is destabilized - slow degradation. Not listed as available.
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[[Future Prospects|Conclusions and Future Work]]
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BBa_E0611: Arabinose turns on destabilized TetR repressor which shuts down yellow fluorescent protein. Addition of Tetracyline blocks TetR repressor allowing yellow fluorescent protein to be turned back on.
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</td>
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BBa_E0601: Like BBa_E0611 but YFP is destabilized - slow degradation. Not listed as available
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<td valign="top" width="50%">
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<h3><ul><li>''' Team 2: Repressilator'''</ul></h3>
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BBa_E0613: Arabinose turns on destabilized lambda repressor which shuts down yellow fluorescent protein.
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Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pLac, hoping that this would assist in synchronizing the oscillations.
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BBa_E0602: Like BBa_E0613 but YFP is destabilized - slow degradation. Not listed as available
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[[Theory Behind the Oscillator|Theory Behind the Oscillator]]
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BBa_E0603: Like BBa_E0602 but baseline expression of YFP is higher. Not listed as available
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[[McGill_Repressilator_M_and_M|Methods and Materials]]
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=Introduction= Our brainstorming meeting on 3-16 gave us several preliminary ideas which we will explore via modeling and research
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[[McGill_Repressilator_Results|Results]]
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</td>
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</tr></table>
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<center><h2><font face="broadway,verdana">Lab Procedures</font></h2></center>
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<table border="0"><tr><td valign="top" width="85%">
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* [[Protocols]]
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* [[Lab Notebook]]
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</td>
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<td>[[Image:Test_tubes.jpg]]</td></tr></table>
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|-valign="top"
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<center><h2><font face="broadway,verdana">Club</font></h2></center>
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* [[News]]
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* [[Team Members]]
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* [[Executive Council|Executive Council]]
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* [[Journal Club Meetings]]
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* [[Journal Club Papers]]
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<center><h2><font face="broadway,verdana">Just for Fun</font></h2></center>
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* [[iGEM Party Pictures]]
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* [[ahessel:montreal|Ambassador Visit]] [http://www.flickr.com/search/?q=igem+montreal&m=text Photos]
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* [[iGEM Soundtrack]]
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<Center>[[Image:Poutine.gif]]</Center>
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__NOTOC__

Latest revision as of 20:19, 31 October 2006

Banner.gif


Welcome to the McGill Wiki! Basically, we're a team of 12 undergrads, 1 grad student, and 1 professor who like to have fun and clone things in our spare time. McGill University is located in Montreal, Quebec, which has given us wonderful opportunities to balance lab work with festivals and general craziness. iGEM has been a great opportunity to get lab experience while having the freedom to be creative, and we look forward to meeting the other teams at the jamboree!

Clip image002.jpgGood 2.jpg



Projects

  • Team 1: Split YFP

This project on fluorescence complementation involves the joining of two proteins, Jun and Fos, each fused to a half terminus of YFP. Both of these chimeric proteins were fused to a beta gene that codes for a membrane protein. Then, two cell populations - one expressing Jun-beta-YFPN and the other Fos-beta-YFPC - were combined, ideally resulting in the fusion of the Jun and Fos proteins on the cell membrane. The two halves of the YFP protein would bind as well, giving rise to fluorescence.

Background

Methods and Materials

Results

Conclusions and Future Work

  • Team 2: Repressilator

Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pLac, hoping that this would assist in synchronizing the oscillations.

Theory Behind the Oscillator

Methods and Materials

Results

Lab Procedures

Test tubes.jpg

Club

Just for Fun

Poutine.gif
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