Brown:Equipment needed

From 2006.igem.org

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{| border=1 cellspacing=0 cellpadding=5
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{| border=1 cellspacing=0 cellpadding=1
|-  
|-  
-
| '''Equipment'''
+
|
-
| | type/size
+
| type/size
| quantity
| quantity
 +
| Have(x)
 +
| Location
 +
| Comments
 +
|-
 +
| '''Equipment'''
 +
|
 +
|
|-  
|-  
| freezers/fridges
| freezers/fridges
Line 12: Line 19:
| -80°C
| -80°C
| 1
| 1
 +
| we have space in a -80
 +
| JWW basement
|-  
|-  
|  
|  
| -20°C
| -20°C
| 1
| 1
 +
| yes
 +
| JWW228
|-  
|-  
-
| | 4°C| 1|-
 
-
| incubator | | 1|-
 
-
| hot bath| | 1|-
 
-
| cold bath| | 1|-
 
-
| autoclave| | 1|-
 
-
| cell shaker/incubator| | 1|-
 
-
| source of ddH2O| | 1|-
 
-
| hot plates w/ stirring| | 2|-
 
-
| stir bars (set)| | 1|-
 
-
| PCR thermocycler| | 1|-
 
-
| microcentrifuge (w/temp cntrl.)| | 1|-
 
-
| centrifuge | 50mL and 15mL| 1|-
 
-
| Electrophoresis gel set| | 4|-
 
-
| Gel Power source| | 1|-
 
-
| UV Gel viewer| | 1|-
 
-
| UV Gel camera| | 1|-
 
-
| Pipettemen| | |-
 
-
| | 20μL| 4|-
 
-
| | 200μL| 4|-
 
-
| | 1000μL| 4|-
 
-
| Electronic pipettor| can use 1-25mL pipettes| 1|-
 
-
| bunsen burners| | 2|-
 
-
| sparkers| | 2|-
 
-
| wire loop| to pick a bac clone| 3|-
 
-
| metal loop| to spread bacteria| 3|-
 
-
| | | |-
 
-
| Glassware| | |-
 
-
| | type/size| quantity|-
 
-
| Bottles| | |-
 
-
| | 1L| 4|-
 
-
| | 500mL| 8|-
 
-
| | 100mL| 8|-
 
-
| Beakers| | |-
 
-
| | 1L| 2|-
 
-
| | 250mL| 8|-
 
-
| Centrifuge tubes| | |-
 
-
| | 50mL| 100|-
 
-
| | 15mL| 100|-
 
-
| Graduated cylinders| | |-
 
-
| | 500mL| 2|-
 
-
| | 100mL| 2|-
 
-
| Electronic pipette tips (packages)| | |-
 
-
| | 1mL| 2|-
 
-
| | 5mL| 2|-
 
-
| | 10mL| 2|-
 
-
| | 25mL| 2|-
 
-
| Gloves (boxes)| | |-
 
-
| | small| 1|-
 
-
| | medium| 4|-
 
-
| | large| 4|-
 
-
| | | |-
 
-
| Plasticware| | |-
 
-
| | | |-
 
-
| centrifuge tubes| | |-
 
-
| | 1.5mL| 2000|-
 
-
| | 200μL (PCR)| 1000|-
 
-
| Pipette tips| | |-
 
-
| | 20μL| a lot|-
 
-
| | 200μL| a lot|-
 
-
| | 1000μL| a lot|-
 
-
| Bacterial cell culture plates| | 200|-
 
-
| bacterial cell culture tubes| | |-
 
-
| | (polypropylene)| 100|-
 
-
| tube racks/holders| | |-
 
-
| | for 1.5mL tubes| 4|-
 
-
| for 200μL tubes| 2|-
 
|  
|  
 +
| 4°C
 +
| 1
 +
| yes
 +
| JWW228
 +
|-
 +
| incubator (what kind?)
|  
|  
-
| Reagents
+
| 1
-
| 70% ethanol
+
| might work (heh)
 +
| JWW228
 +
|-
 +
| pH meter
|  
|  
 +
| 1
 +
|
 +
|-
 +
| hot bath
 +
|
 +
| 1
 +
| we have access
 +
| JWW218
 +
|-
 +
| cold bath
 +
|
 +
| 1
 +
|-
 +
| autoclave
 +
|
 +
| 1
 +
| we have access
 +
|JWW 4th Floor
 +
|
 +
|-
 +
| cell shaker inside 37 degree incubator
 +
|
 +
| 1
 +
| we have acess
 +
| JWW 3rd floor
 +
|-
 +
| source of ddH2O
 +
|
 +
| 1
 +
|-
 +
| hot plates w/ stirring
 +
|
 +
| 2
 +
|-
 +
| stir bars (set)
 +
|
 +
| 1
 +
|-
 +
| PCR thermocycler
 +
|
 +
| 1
 +
|-
 +
| microcentrifuge (w/temp cntrl.)
 +
| temp. cntrl not essential
 +
| 1
 +
|-
 +
| centrifuge
 +
| 50mL and 15mL
 +
| 1
 +
| yes - 15 mL
 +
| very old, JWW228
 +
|-
 +
| Electrophoresis gel set
 +
|
 +
| 4
 +
|-
 +
| Gel Power source
 +
|
 +
| 1
 +
|-
 +
| UV Gel viewer
 +
|
 +
| 1
 +
|-
 +
| UV Gel camera
 +
|
 +
| 1
 +
|-
 +
| Pipettemen
 +
|
 +
|
 +
|-
 +
|
 +
| 20μL
 +
| 4
 +
|-
 +
|
 +
| 200μL
 +
| 4
 +
|-
 +
|
 +
| 1000μL
 +
| 4
 +
|-
 +
| Electronic pipettor
 +
| can use 1-25mL pipettes
 +
| 1
 +
|-
 +
| Manual pipettors / bulbs
 +
|
 +
| 2
 +
|-
 +
| bunsen burner
 +
|
 +
| 2
 +
|-
 +
| sparkers
 +
|
 +
| 2
 +
|-
 +
| wire loop
 +
| to pick a bac clone
 +
| 3
 +
|-
 +
| metal loop
 +
| to spread bacteria
 +
| 3
 +
|- 
 +
| '''Glassware'''
 +
|
 +
|
 +
|-
 +
| Bottles
 +
|
 +
|
 +
|-
 +
|
 +
| 1L
 +
| 4
 +
|-
 +
|
 +
| 500mL
 +
| 8
 +
|-
 +
|
 +
| 100mL
 +
| 8
 +
|-
 +
| Beakers
 +
|
 +
|
 +
|-
 +
|
 +
| 1L
 +
| 2
 +
|-
 +
|
 +
| 250mL
 +
| 8
 +
|-
 +
| Centrifuge tubes
 +
|
 +
|
 +
|-
 +
|
 +
| 50mL
 +
| 100
 +
|-
 +
|
 +
| 15mL
 +
| 100
 +
|-
 +
| Graduated cylinders
 +
|
 +
|
 +
|-
 +
|
 +
|
 +
500mL
 +
| 2
 +
|-
 +
|
 +
| 100mL
 +
| 2
 +
|-
 +
| Electronic pipette tips (packages)
 +
|
 +
|
 +
|-
 +
|
 +
| 1mL
 +
| 2
 +
|-
 +
|
 +
| 5mL
 +
| 2
 +
|-
 +
|
 +
| 10mL
 +
| 2
 +
|-
 +
|
 +
| 25mL
 +
| 2
 +
|-
 +
| pasteur pipettes
 +
|
 +
| 1 box
 +
|-
 +
| '''Gloves (boxes)'''
 +
|
 +
|
 +
|-
 +
|
 +
| small
 +
| 1
 +
|-
 +
|
 +
| medium
 +
| 4
 +
|-
 +
|
 +
| large
 +
| 4
 +
|-
 +
| '''Plasticware'''
 +
|
 +
|
 +
|-
 +
| eppendorf tubes
 +
|
 +
|
 +
|-
 +
|
 +
| 1.5mL
 +
| 2000
 +
|-
 +
|
 +
| 200μL (thin wall for PCR)
 +
| 1000
 +
|-
 +
| Pipette tips
 +
|
 +
|
 +
|-
 +
|
 +
| 20μL
 +
| a lot
 +
|-
 +
|
 +
| 200μL
 +
| a lot
 +
|-
 +
|
 +
| 1000μL
 +
| a lot
 +
|-
 +
| Bacterial cell culture plates
 +
|
 +
| 200
 +
|-
 +
| bacterial cell culture tubes
 +
| (polypropylene)
 +
| 100
 +
|-
 +
| tube racks/holders
 +
|
 +
|
 +
|-
 +
|
 +
| for 1.5mL tubes
 +
| 4
 +
|-
 +
|
 +
| for 200μL tubes
 +
| 2
 +
|-
 +
| '''Reagents'''
 +
|
 +
|
 +
|-
 +
| pH standard buffers for meter
 +
|
 +
| 1
 +
|-
 +
| 70% ethanol
 +
|
 +
|
 +
|-
 +
| anitibiotics
 +
|
 +
|
 +
|-
 +
|
 +
| ampicillin
 +
|
 +
|-
 +
|
 +
| kanamycin
 +
|
 +
|-
 +
|
 +
| puromycin
 +
|
 +
|-
 +
| Agarose
 +
|
 +
|
 +
|-
 +
| Ethidium Bromide
 +
|
 +
|
 +
|-
 +
| tris[hydroxymethyl]aminomethane
 +
|
 +
|
 +
|-
 +
| Acetate
 +
|
 +
|
 +
|-
 +
| EDTA
 +
|
 +
|
 +
|-
 +
| Gel Loading Dye
 +
|
 +
|
 +
|-
 +
| DNA ladder
 +
|
 +
|
 +
|-
 +
| Restriction enzymes and Buffers
 +
|
 +
|
 +
|-
 +
|
 +
| EcoRI
 +
|
 +
|-
 +
|
 +
| SpeI
 +
|
 +
|-
 +
|
 +
| XbaI
 +
|
 +
|-
 +
|
 +
| PstI
 +
|
 +
|-
 +
| Taq Polymerase (Master Mix)
 +
|
 +
|
 +
|-
 +
| 100% ethanol
 +
|
 +
|
 +
|-
 +
| Socium Acetate
 +
|
 +
|
 +
|-
 +
| NaCl
 +
|
 +
|
 +
|-
 +
| T4 DNA ligase
 +
|
 +
|
 +
|-
 +
| T4 DNA ligase Buffer
 +
|
 +
|
 +
|-
 +
| liquid LB Broth
 +
|
 +
|
 +
|-
 +
| LB with agar
 +
|
 +
|
 +
|-
 +
| TSS Buffer
 +
|
 +
|
 +
|-
 +
| Mini-Prep Kit
 +
|
 +
|
 +
|-
 +
| Maxi-Prep Kit
 +
|
 +
|
 +
|-
|}
|}
-
 
-
      $ 400.00  Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>  custom primers 
 
-
      $ 800.00  Antibodies for identifying cell division proteins 4 x $200 
 
-
      $ 250.00  disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates) 
 
-
      $ 600.00  Gel kit: Agarose gel aparatus, power source 
 
-
          TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros 
 
-
          Taq polymerase kit, DNTP - Free bases (actg ready mixed) 
 
-
      $ 600.00  Restriction enzymes  $60 x 10 
 
-
      $ 300.00  Thermocycler 
 
-
      $ 500.00  misc. equipment and supplies 
 
-
      $ 4,000.00  DNA synthesis costs $1.40 per base. 
 
-
        Protein coding regions are about 700 to 1000 bases long  This budget is for 3 - 4 new proteins.
 

Latest revision as of 16:50, 19 June 2006

type/size quantity Have(x) Location Comments
Equipment
freezers/fridges
-80°C 1 we have space in a -80 JWW basement
-20°C 1 yes JWW228
4°C 1 yes JWW228
incubator (what kind?) 1 might work (heh) JWW228
pH meter 1
hot bath 1 we have access JWW218
cold bath 1
autoclave 1 we have access JWW 4th Floor
cell shaker inside 37 degree incubator 1 we have acess JWW 3rd floor
source of ddH2O 1
hot plates w/ stirring 2
stir bars (set) 1
PCR thermocycler 1
microcentrifuge (w/temp cntrl.) temp. cntrl not essential 1
centrifuge 50mL and 15mL 1 yes - 15 mL very old, JWW228
Electrophoresis gel set 4
Gel Power source 1
UV Gel viewer 1
UV Gel camera 1
Pipettemen
20μL 4
200μL 4
1000μL 4
Electronic pipettor can use 1-25mL pipettes 1
Manual pipettors / bulbs 2
bunsen burner 2
sparkers 2
wire loop to pick a bac clone 3
metal loop to spread bacteria 3
Glassware
Bottles
1L 4
500mL 8
100mL 8
Beakers
1L 2
250mL 8
Centrifuge tubes
50mL 100
15mL 100
Graduated cylinders

500mL

2
100mL 2
Electronic pipette tips (packages)
1mL 2
5mL 2
10mL 2
25mL 2
pasteur pipettes 1 box
Gloves (boxes)
small 1
medium 4
large 4
Plasticware
eppendorf tubes
1.5mL 2000
200μL (thin wall for PCR) 1000
Pipette tips
20μL a lot
200μL a lot
1000μL a lot
Bacterial cell culture plates 200
bacterial cell culture tubes (polypropylene) 100
tube racks/holders
for 1.5mL tubes 4
for 200μL tubes 2
Reagents
pH standard buffers for meter 1
70% ethanol
anitibiotics
ampicillin
kanamycin
puromycin
Agarose
Ethidium Bromide
tris[hydroxymethyl]aminomethane
Acetate
EDTA
Gel Loading Dye
DNA ladder
Restriction enzymes and Buffers
EcoRI
SpeI
XbaI
PstI
Taq Polymerase (Master Mix)
100% ethanol
Socium Acetate
NaCl
T4 DNA ligase
T4 DNA ligase Buffer
liquid LB Broth
LB with agar
TSS Buffer
Mini-Prep Kit
Maxi-Prep Kit
Retrieved from "http://2006.igem.org/wiki/index.php/Brown:Equipment_needed"
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