From 2006.igem.org
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| - | # Heat Unstable Nucleoid Protein
| + | List of Plasmid Biobricks[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=plasmid_biobrick&show=1] |
| - | # HU stimulates trascription using lambda phage template
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| - | # It is made of 2 subunits: HU alpha and HU beta
| + | GFP[http://partsregistry.org/Part:BBa_E0040] |
| - | # It is an abundant non-specific DNA binding protein which is highly conserved between prokaryotes
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| - | # About 30,000 to 55,000 HU molecules per cell exist in the exponentially growing cells of E. coli W3110. Indicating that HU, together with Fis, forms a major nucleoid component in the growing E. coli cell
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| - | # Considered prokaryotic homolog of eukaryotic histones which restrains DNA supercoils in the nucleoid
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| - | # Certain fraction of HU is associated with ribosomes
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| - | # At saturation, HU dimers may be associated, on average, every 300 to 400 bp of the E. coli genome
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| - | # Upon entry into the stationary phase, the HU level started to decrease gradually, decreasing to less than one-third of the maximum level in the late stationary phase
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Latest revision as of 16:30, 30 May 2006
List of Plasmid Biobricks[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=plasmid_biobrick&show=1]
GFP[http://partsregistry.org/Part:BBa_E0040]
