File registry
From 2006.igem.org
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| + | [[Lab_Notebook|Lab Notebook]] | ||
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'''File Registry''':Good Afternoon Everyone, I started this page to register our Plasmids Minipreps, Etc. to provide additional information other than just what's on the Sheet. | '''File Registry''':Good Afternoon Everyone, I started this page to register our Plasmids Minipreps, Etc. to provide additional information other than just what's on the Sheet. | ||
'''Complete Plasmids Box''': | '''Complete Plasmids Box''': | ||
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| + | '''1A''': '''IT WORKS!!!''' A digestion was done June 1, confirms this Midiprep. This is a '''MIDIPREP''' of '''pGFP''' that was performed on '''Monday May 29''' by Adam and Ashwin. This midiprep combined two seedings that were performed on the prior weekend. Both have been ran through the same midiprep column. The plasmid DNA here should be extremely concentrated as the maximm 50 ml of Culture was used. The total dissolving volume here is 200uL of dH2O. Unfortunately some product may hjave been lost during the last step of transferring the product from a big to a small centrifuge tube. | ||
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| + | '''1B''': '''NO LONGER GOOD'''. This is a '''MINIPREP''' product of '''MscL plasmid''' purified from a culture of '''Stable 3''' bacteria by Jieun, Aaron and Brock on '''Tuesday May 30'''. The way this was done is a 5ml culture of Stable 3 was divided into 5 tubes and then pooled into 2 final product tubes, 1B and 1C. The DNA in this tube was initially dissolved in 50 uL of water. 15 uL of this DNA was removed for a restriction digest by XmnI on the same day, May 30 leaving 35um. Unfortunately the soluution is no longer good because 2um XmnI was accidentally added to this tube. | ||
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| + | '''1C''': Like 1B, this is the other half of the '''MINIPREP''' product of '''MscL plasmid''' purified from a culture of '''Stable 3''' bacteria by Jieun, Aaron and Brock on '''Tuesday May 30'''. Please refer to '''1B''' for the way this was done. It was dissolved in 50uL of dH2O and as of this date, nothing was removed from this solution. It is believed to be '''STILL GOOD'''. '''The Digestion Gel has Shown that this miniprep contains correct DNA but faint''' | ||
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| + | '''1D''': This is a '''MINIPREP''' product of '''MscL plasmid''' purified from a culture of '''DH5alpha''' bacteria by Ashwin, Alex, and Adam on '''Tuesday May 30'''. It was done the same way as 1B and 1C. 5 ml cultuire was used, spilt to 5 tubes and then combined t 2 final tubes 1D and 1E. Each was dissolved in a total voulme of 50um dH2O. 15um of this solution was taken for a restriction digest leaving 35uL total as of current date. '''The Digestion Gel has Shown that this miniprep contains correct DNA but faint''' | ||
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| + | '''1E''':This is a '''MINIPREP''' product of '''MscL plasmid''' purified from a culture of '''DH5alpha''' bacteria by Ashwin, Alex, and Adam on '''Tuesday May 30'''. It was done the same way as 1B and 1C. 5 ml cultuire was used, spilt to 5 tubes and then combined t 2 final tubes 1D and 1E. Each was dissolved in a total voulme of 50um dH2O. As of current date none of this solution has been removed. '''The Digestion Gel has Shown that this miniprep contains correct DNA but faint''' | ||
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| + | '''1F''': This is '''BBa_I5610''' which is in a '''pSB1A2''' plasmid. There is 8 um of this solution, and it was dissolved by Aaron and Josh on '''Thursday, June 1'''. | ||
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| + | '''1G''': This is '''BBa_I15004''' which is in a '''pSB3K3''' plasmid. There is 8 um of this solution and it was dissolved by Jamie and Josh on '''Friday, June 2'''. | ||
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| + | '''1H''': This is '''BBa_R0062''' which is in a '''pSB1A2''' plasmid. There is 8 um of this solution and it was dissolved by Jamie and Josh on '''Friday, June 2'''. | ||
Latest revision as of 17:50, 12 July 2006
File Registry:Good Afternoon Everyone, I started this page to register our Plasmids Minipreps, Etc. to provide additional information other than just what's on the Sheet.
Complete Plasmids Box:
1A: IT WORKS!!! A digestion was done June 1, confirms this Midiprep. This is a MIDIPREP of pGFP that was performed on Monday May 29 by Adam and Ashwin. This midiprep combined two seedings that were performed on the prior weekend. Both have been ran through the same midiprep column. The plasmid DNA here should be extremely concentrated as the maximm 50 ml of Culture was used. The total dissolving volume here is 200uL of dH2O. Unfortunately some product may hjave been lost during the last step of transferring the product from a big to a small centrifuge tube.
1B: NO LONGER GOOD. This is a MINIPREP product of MscL plasmid purified from a culture of Stable 3 bacteria by Jieun, Aaron and Brock on Tuesday May 30. The way this was done is a 5ml culture of Stable 3 was divided into 5 tubes and then pooled into 2 final product tubes, 1B and 1C. The DNA in this tube was initially dissolved in 50 uL of water. 15 uL of this DNA was removed for a restriction digest by XmnI on the same day, May 30 leaving 35um. Unfortunately the soluution is no longer good because 2um XmnI was accidentally added to this tube.
1C: Like 1B, this is the other half of the MINIPREP product of MscL plasmid purified from a culture of Stable 3 bacteria by Jieun, Aaron and Brock on Tuesday May 30. Please refer to 1B for the way this was done. It was dissolved in 50uL of dH2O and as of this date, nothing was removed from this solution. It is believed to be STILL GOOD. The Digestion Gel has Shown that this miniprep contains correct DNA but faint
1D: This is a MINIPREP product of MscL plasmid purified from a culture of DH5alpha bacteria by Ashwin, Alex, and Adam on Tuesday May 30. It was done the same way as 1B and 1C. 5 ml cultuire was used, spilt to 5 tubes and then combined t 2 final tubes 1D and 1E. Each was dissolved in a total voulme of 50um dH2O. 15um of this solution was taken for a restriction digest leaving 35uL total as of current date. The Digestion Gel has Shown that this miniprep contains correct DNA but faint
1E:This is a MINIPREP product of MscL plasmid purified from a culture of DH5alpha bacteria by Ashwin, Alex, and Adam on Tuesday May 30. It was done the same way as 1B and 1C. 5 ml cultuire was used, spilt to 5 tubes and then combined t 2 final tubes 1D and 1E. Each was dissolved in a total voulme of 50um dH2O. As of current date none of this solution has been removed. The Digestion Gel has Shown that this miniprep contains correct DNA but faint
1F: This is BBa_I5610 which is in a pSB1A2 plasmid. There is 8 um of this solution, and it was dissolved by Aaron and Josh on Thursday, June 1.
1G: This is BBa_I15004 which is in a pSB3K3 plasmid. There is 8 um of this solution and it was dissolved by Jamie and Josh on Friday, June 2.
1H: This is BBa_R0062 which is in a pSB1A2 plasmid. There is 8 um of this solution and it was dissolved by Jamie and Josh on Friday, June 2.
