Plasmids and Bacteria
From 2006.igem.org
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* Transform bacteria '''We are using ''Salmonella typhimurium'' for the Hin protein as it was the referenced species in all of the literature.''' | * Transform bacteria '''We are using ''Salmonella typhimurium'' for the Hin protein as it was the referenced species in all of the literature.''' | ||
* Create RE biobrick (completed) | * Create RE biobrick (completed) | ||
| - | + | *Isolating genomic DNA from Salmonella typhimurium (completed) | |
| - | *Isolating genomic DNA from Salmonella typhimurium | + | |
*Ordered oligos for recombinational enhancer and primers for the hin gene | *Ordered oligos for recombinational enhancer and primers for the hin gene | ||
| + | **These sequences will be transformed into RFP and possibly GFP plasmids | ||
| + | *Performed Minipreps to isolate RFP and GFP plasmids to be used for transformations. | ||
Latest revision as of 17:17, 8 June 2006
- Transform bacteria We are using Salmonella typhimurium for the Hin protein as it was the referenced species in all of the literature.
- Create RE biobrick (completed)
- Isolating genomic DNA from Salmonella typhimurium (completed)
- Ordered oligos for recombinational enhancer and primers for the hin gene
- These sequences will be transformed into RFP and possibly GFP plasmids
- Performed Minipreps to isolate RFP and GFP plasmids to be used for transformations.
