Hin gene: Part BBa J31000

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'''''Hin gene: Part BBa_J31000'''''
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'''''Hin gene: Part BBa_J31000'''''[http://partsregistry.org/Part:BBa_J31000]
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Primers for Hin gene the 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in '''bold'''.
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Primers for Hin gene
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* The 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in <font color='magenta'>pink</font color>.
'''Forward primer'''
'''Forward primer'''
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*<font color=green>Consists of four extra base pairs</font color>, <font color=red>XBA restriction site</font color>, <font color=blue> and the first 85 base pairs of the [http://partsregistry.org/Part:BBa_J31000| Hin gene]</font color>
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5’ gcattctagaatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttac'''C'''agtgcaaattg 3’
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'''<font face="courier new">5’ <font color='green'>GCAT</font color><font color=red>TCTAGA</font color><font color=blue>ATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTAC<font color='magenta'>C</font color>AGTGCAAATTG</font color> 3’</font face>'''
'''Reverse primer'''
'''Reverse primer'''
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*Consists of <font color=green> four extra base pairs</font color>,<font color=red> Bio Brick</font color>, <font color=orange> the last 23 base pairs of the Hin gene</font color>
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5’ atgcctgcaggcggccgcactagtttaattcattcgtttttttatac 3’
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'''<font face="courier new">5’<font color=green> ATGC</font color><font color=red>CTGCAGGCGGCCGCACTAGT</font color><font color=orange>TTAATTCATTCGTTTTTTTATAC</font color> 3’</font face>'''

Latest revision as of 21:04, 12 June 2006

Hin gene: Part BBa_J31000[http://partsregistry.org/Part:BBa_J31000]

Primers for Hin gene

  • The 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in pink.

Forward primer

  • Consists of four extra base pairs, XBA restriction site, and the first 85 base pairs of the [http://partsregistry.org/Part:BBa_J31000| Hin gene]

5’ GCATTCTAGAATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTACCAGTGCAAATTG 3’

Reverse primer

  • Consists of four extra base pairs, Bio Brick, the last 23 base pairs of the Hin gene

5’ ATGCCTGCAGGCGGCCGCACTAGTTTAATTCATTCGTTTTTTTATAC 3’

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