SuperParts
From 2006.igem.org
(Difference between revisions)
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| + | Western Assembly Plan | ||
| + | |||
| + | Parts List | ||
| + | pLac promoter -- BBa_R0010 DNA-1 7K | ||
| + | pAra promoter -- BBa_I13453 DNA-2 13D | ||
| + | RBS -- BBa_B0030 DNA-1 3G | ||
| + | HixC – BBa_B????? | ||
| + | double forward terminator -- BBa_B0015 DNA-1 1I | ||
| + | double backward terminator – BBa_B0025 DNA-1 3E | ||
| + | KanR – BBa_B????? | ||
| + | Recombination enhancer -- BBa_J3101 (Davidson) | ||
| + | Hin -- BBa_J31000 (Davidson) | ||
| + | pAra promoter reverse – BBa_B????? (Western) | ||
| + | RBS reverse – BBa_B????? (Western) | ||
| + | |||
| + | Upon receipt of the Hin and HixC from Davidson, Western will assemble the following Hin Expression Unit, both with and without an additional HixC, and send some of the finished product to Davidson (specifically that without the HixC site). | ||
| + | |||
| + | |||
[[Image:HinExpressionUnitJPEG.jpg]] | [[Image:HinExpressionUnitJPEG.jpg]] | ||
Revision as of 00:13, 30 June 2006
Western Assembly Plan
Parts List pLac promoter -- BBa_R0010 DNA-1 7K pAra promoter -- BBa_I13453 DNA-2 13D RBS -- BBa_B0030 DNA-1 3G HixC – BBa_B????? double forward terminator -- BBa_B0015 DNA-1 1I double backward terminator – BBa_B0025 DNA-1 3E KanR – BBa_B????? Recombination enhancer -- BBa_J3101 (Davidson) Hin -- BBa_J31000 (Davidson) pAra promoter reverse – BBa_B????? (Western) RBS reverse – BBa_B????? (Western)
Upon receipt of the Hin and HixC from Davidson, Western will assemble the following Hin Expression Unit, both with and without an additional HixC, and send some of the finished product to Davidson (specifically that without the HixC site).
