Induction
From 2006.igem.org
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[[McGill_University_2006|Home]] <br> | [[McGill_University_2006|Home]] <br> | ||
[[Protocols|Protocols]] | [[Protocols|Protocols]] | ||
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| + | 1.Grow cultures to mid-log stage (O.D. ~3.0) | ||
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| + | 2.Add 1uL of IPTG per mL of solution (gives 1mM conc.) | ||
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| + | 3.Place in S-I for another hour or two. | ||
Latest revision as of 23:15, 13 July 2006
1.Grow cultures to mid-log stage (O.D. ~3.0)
2.Add 1uL of IPTG per mL of solution (gives 1mM conc.)
3.Place in S-I for another hour or two.
