ETH 2006 Half adder
From 2006.igem.org
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== Idea: Pattern recognition == | == Idea: Pattern recognition == | ||
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| + | * [[media:Half-adder.pdf|sliles]] (pdf, 352KB) | ||
| + | * [[media:Half-adder-handout.pdf|handout]] (pdf, 268KB) | ||
===Main Goal:=== | ===Main Goal:=== | ||
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**Bacteria does not express fluorescent protein when pattern on the plate and picture match (no light and no chemical) | **Bacteria does not express fluorescent protein when pattern on the plate and picture match (no light and no chemical) | ||
**Bacteria gets red when pattern on the plate and picture do not match | **Bacteria gets red when pattern on the plate and picture do not match | ||
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====Implementation from the ingenieur point of view==== | ====Implementation from the ingenieur point of view==== | ||
Revision as of 11:29, 31 July 2006
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Contents |
Half-adder or Full-adder
An implementation of a half-adder or full-adder: 1-bit adder with carry
- [http://en.wikipedia.org/wiki/Full_adder half/full adder in wikipedia]
Idea: Pattern recognition
→ Illustration of the concepts:
Main Goal:
- Write something with a chemical on a petri plate (like ETH for example)
- Let Bacteria grow uniformly on the plate
- Expose the plate to a picture (black and white) of the same pattern
- Result:
- Bacteria gets green when pattern on the plate and picture match (light and chemical)
- Bacteria does not express fluorescent protein when pattern on the plate and picture match (no light and no chemical)
- Bacteria gets red when pattern on the plate and picture do not match
Implementation from the ingenieur point of view
- Half Adder
- = opportunity to build an AND and an XOR Gate
Implementation from the biologist point of view
- AND Gate: presence of chemical induces the production of an inactive protein, which is activated by a second protein (produced when light is present): the first protein is then activating GFP production
- XOR Gate: I think Marko had a good idea about that (I didn't really got it)
- a light sensible promoter (there are some)
- a chemical sensible promoter (chemical to be defined)
- we would need to give a kind of first signal to the system (to don't have light reactions before a certain moment, otherwise we have to work in a dark room...), an activation signal after the bacterias have grown on the plate
Pro's & Con's
Pro's:
- meaningful from engineering point
- valuable parts for synthetic biology
- experiments visually attractive
- probably simple enough
Con's:
- cheap copy of “bio-film” project (iGEM 2004)
- sensational experiments, have little in common with HA
- too simple?
