Pancake Parts

From 2006.igem.org

Revision as of 17:21, 2 August 2006 (view source) Erzwack (Talk | contribs) ← Older edit		Revision as of 17:25, 2 August 2006 (view source) Erzwack (Talk | contribs) Newer edit →
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	*KanR Back - still trying to clone		*KanR Back - still trying to clone
	[[Construction Intermediates]]		[[Construction Intermediates]]
		+	*Pbad-RBS - used culture 3
		+	*Hix-TF-Hix-TT-RE - used culture 1
		+	*Pbad-Hix-RBS - used culture 3
		+	*TF-Hix-TT-RE - used culture 2
		+	*
	[[One Pancake Constructs]]		[[One Pancake Constructs]]

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Revision as of 17:25, 2 August 2006

Hin gene: Part BBa_J31000

• Used Hin 100-1

Primers for Hin gene with LVA degredation tag

• Used HinLVA-2

Recombinational Enahancer: Part BBa_J3101

• used RE-11

Hix- Part BBa_J44000[http://partsregistry.org/Part:BBa_J44000]

• Used Hix C-2

Resistance Genes

• TetR Front - used TF10
• TetR Back- used TB5
• CmR Front - used CF4
• CmR Back - used CB8
• KanR Front - used KF7
• KanR Back - still trying to clone

Construction Intermediates

• Pbad-RBS - used culture 3
• Hix-TF-Hix-TT-RE - used culture 1
• Pbad-Hix-RBS - used culture 3
• TF-Hix-TT-RE - used culture 2

One Pancake Constructs