Ljubljana, Slovenia 2006/Project
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Although in the past years all participating iGEM teams focused on bacteria and yeast, we decided to modify signalling pathways in higher eukaryotes. This is a far more complicated system to understand but we feel that the recently accumulated knowledge enables rational planning of cells with novel properties. | Although in the past years all participating iGEM teams focused on bacteria and yeast, we decided to modify signalling pathways in higher eukaryotes. This is a far more complicated system to understand but we feel that the recently accumulated knowledge enables rational planning of cells with novel properties. | ||
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| + | == Background == | ||
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| + | When bacteria attack a human body, immune response is the normal reaction of the organism. Cells which represent the first line of defence are macrophages and the way they act is rather complicated. On the surface of these cells (actually inserted into cell membrane) are several types of receptor molecules, commonly abbreviated as TLR (toll-like receptors). These can specifically bind various components of bacteria (e.g. lipopolysaccharides which are part of bacterial outer membranes) and upon binding, they interact with other molecules inside the cell, forming a cell signalling pathway that, after several relays, ends in the cell nucleus and induces production of immune response proteins, such as cytokines and chemokines. The complete pathway is depicted [http://www.komabiotech.co.kr/technical/review/toll_like_receptor.gif here] and is rather complicated. Stages of the signal transfer were elucidated recently. Cell signalling like this is the basis of our efficient innate immune response (i.e. the fast-acting, not mediated by antibodies) against microbes. In some instances, however, signalling mediated by TLR is not beneficial. Under some not yet fully understood circumstances, bacteria can trigger this same signalling pathway which results in excessive inflamatory response. As result, sepsis occurs, which can lead to organ failure and in about 20% cosequences are fatal. | ||
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| + | == Signalling pathway == | ||
| + | After binding of bacterial constituents to TLR, its intracellular TIR-domain interacts with either MyD88 or TRIF, two signalling molecules in the cytoplasm that relay signal to other components of the pathway as shown [http://www.biken.osaka-u.ac.jp/act/images/akiraB.png here]. | ||
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| + | == Idea == | ||
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| + | == Approach == | ||
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| + | == Anticipated results == | ||
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| + | ---- | ||
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''(more to follow)'' | ''(more to follow)'' | ||
[[University of Ljubljana & National Institute of Chemistry, Ljubljana, Slovenia 2006|Back home]] | [[University of Ljubljana & National Institute of Chemistry, Ljubljana, Slovenia 2006|Back home]] | ||
Revision as of 20:23, 13 August 2006
Although in the past years all participating iGEM teams focused on bacteria and yeast, we decided to modify signalling pathways in higher eukaryotes. This is a far more complicated system to understand but we feel that the recently accumulated knowledge enables rational planning of cells with novel properties.
Contents |
Background
When bacteria attack a human body, immune response is the normal reaction of the organism. Cells which represent the first line of defence are macrophages and the way they act is rather complicated. On the surface of these cells (actually inserted into cell membrane) are several types of receptor molecules, commonly abbreviated as TLR (toll-like receptors). These can specifically bind various components of bacteria (e.g. lipopolysaccharides which are part of bacterial outer membranes) and upon binding, they interact with other molecules inside the cell, forming a cell signalling pathway that, after several relays, ends in the cell nucleus and induces production of immune response proteins, such as cytokines and chemokines. The complete pathway is depicted [http://www.komabiotech.co.kr/technical/review/toll_like_receptor.gif here] and is rather complicated. Stages of the signal transfer were elucidated recently. Cell signalling like this is the basis of our efficient innate immune response (i.e. the fast-acting, not mediated by antibodies) against microbes. In some instances, however, signalling mediated by TLR is not beneficial. Under some not yet fully understood circumstances, bacteria can trigger this same signalling pathway which results in excessive inflamatory response. As result, sepsis occurs, which can lead to organ failure and in about 20% cosequences are fatal.
Signalling pathway
After binding of bacterial constituents to TLR, its intracellular TIR-domain interacts with either MyD88 or TRIF, two signalling molecules in the cytoplasm that relay signal to other components of the pathway as shown [http://www.biken.osaka-u.ac.jp/act/images/akiraB.png here].
Idea
Approach
Anticipated results
(more to follow)
