Minutes
From 2006.igem.org
(Difference between revisions)
| Line 27: | Line 27: | ||
'''Holidays''' | '''Holidays''' | ||
| - | * Jude **8<sup>th</sup> – 16<sup>th</sup> August | + | * Jude |
| + | **8<sup>th</sup> – 16<sup>th</sup> August | ||
* Laszlo 8<sup>th</sup> – 22<sup>nd</sup> August | * Laszlo 8<sup>th</sup> – 22<sup>nd</sup> August | ||
* Sree 24<sup>th</sup> – 25<sup>th</sup> August | * Sree 24<sup>th</sup> – 25<sup>th</sup> August | ||
Revision as of 14:39, 8 August 2006
Contents |
Week 7: 31st July - 4th August
Meeting - 2nd August
Wiki
- Update Wiki re. primers for biobricks
Tamara’s visit
- Team meeting at 10am in Hons room on Tuesday 8th August
- Construct an agenda for meeting
- Show her round the labs
- Attend a Fringe Festival show in the evening
Cambridge visit
- Best dates are 22nd to 24th August
- Failing this, the start of September is okay
- Jude currently has 3 spare rooms (possibly 4) from 22nd to 24th August
- Invite ICL as well
Holidays
- Jude
- 8th – 16th August
- Laszlo 8th – 22nd August
- Sree 24th – 25th August
- Jelena 27th August – 9th September
- Kim 8th – 28th September
- Farid ??? September
- Bryony ??? September
pH Turtle
- Currently not working. Chris suggests deleting mouse software from laptop. Will try this.
Modelling
- Meeting on Friday 4th August with Hong Wu
Practical construction of working device
- Kim has come up with lots of characterization experiments to be done in the lab next week once we have the arsenic-sensing device
- Device might be ready on Friday 4th August
- Email Andrew Meharg (author of Venomous Earth) to ask his advice on arsenic biosensing device
Website
- Do we need a disclaimer?
- Need to design Macteria mascot – possibly ask the guy who did the cartoon for Drew and Endy (Nature website)!
Biobricks
- These can be added to the registry when they are ready (well done Jude who has done this before the minutes have been typed!)
Week 5: 17th - 21st July
Meeting - 18th July
Labwork on Arsenic Biosensor:
Biobricks
- PCR on arsenic and LacZ parts
- 4 bands on gel from LacZ so need to find out which one is the correct one
- Create sticky ends
- Working towards arsenic device – may be ready in about 2 weeks
pH Experiments
- Want to measure population density at the point at which pH of blue and white E-Coli diverges
- Difference between stationary and growth phase response is possibly due to the fact that the bacteria ‘want’ to use up the lactose when they’re in the growth phase
- Ideal conditions for growth in LB have been established
- Want to carry out experiments using different growth media
Model
- Plenty of info on Lac operon
- Paper found regarding ArsR relation to arsenic concentration
- Autoregulation – ArsR represses itself
- Background noise may be caused due to degradation of ArsR repressor
- Might be possible to reduce this background noise by separating the promoter from the ArsR gene
- Need to calculate what pH from urea breakdown will be for different concentrations
- It is thought to be necessary to have the two-part promoter (with activator and repressor binding sites) in order to prevent a build up of alkalinity which could harm the bacteria
Wiki
- Update biobricks
Cambridge trip
- Train leaves Edinburgh Waverley at 9.30am so try to be there by about 9am
- Need to prepare a presentation (half an hour long)
- Update animation of arsenic biosensor
- Focus is arsenic project
- Mention 3D structure builder briefly
- Presentation layout
- Background – Jelena
- Building biobricks – Jude
- pH experiment/animation – Kim
- Modelling – Patrick
Idea 6/11 – 3D structure builder
- Primer design for urease
- LacI biobrick – is it ok?
To do
- Moving all experiments to Andrew Miller’s lab
- Meeting on Thursday at 2pm in Honours Room
- Website development
- Design a badge if you have a spare five minutes
- Presentation completed
