Balloon Bacteria : Chiba 2006
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[[Image:Balloonconcept.jpg]] | [[Image:Balloonconcept.jpg]] | ||
- | + | The light in the morning leaking from a gap between the curtains... this morning lights calls and awakes the E. coli. | |
- | + | Suprise!! | |
+ | |||
+ | The E. coli become bigger and bigger like BALLOON!!! | ||
+ | |||
+ | '''→World first bacteria that swells by lights.!!!''' | ||
+ | |||
+ | |||
+ | =Design= | ||
+ | |||
+ | to stop the e.coli by light, we needed a part that stop the e.coli, and combine this with a light receptor that UCSF made it last year. | ||
+ | |||
+ | there was some ways to stop the e.coli running: | ||
+ | #making a chemaera with chemotaxis receptor and light sensing receptor | ||
+ | #stop the motor protein's expression(motB protein: from last year's parts) | ||
+ | #controling chemotaxis | ||
+ | etc | ||
+ | |||
+ | we chose to control chemotaxis. | ||
+ | We've found out, from the paper(..see refrences), the chemoreceptor that fix the e.coli tumble. | ||
+ | it is a fragment of the chemoreceptor "Tsr" (a serin receptor)from aa 290 to 551. we call this tsr-cw(or tsr290). | ||
+ | |||
+ | <br /> | ||
+ | [[Image:pathway.jpg|pathway]] | ||
Revision as of 07:49, 30 October 2006
Concept
The light in the morning leaking from a gap between the curtains... this morning lights calls and awakes the E. coli.
Suprise!!
The E. coli become bigger and bigger like BALLOON!!!
→World first bacteria that swells by lights.!!!
Design
to stop the e.coli by light, we needed a part that stop the e.coli, and combine this with a light receptor that UCSF made it last year.
there was some ways to stop the e.coli running:
- making a chemaera with chemotaxis receptor and light sensing receptor
- stop the motor protein's expression(motB protein: from last year's parts)
- controling chemotaxis
etc
we chose to control chemotaxis. We've found out, from the paper(..see refrences), the chemoreceptor that fix the e.coli tumble. it is a fragment of the chemoreceptor "Tsr" (a serin receptor)from aa 290 to 551. we call this tsr-cw(or tsr290).
Member
Chiba 2006 http://chem.tf.chiba-u.jp/igem/ iGEM Chiba
『je』team