Balloon Bacteria : Chiba 2006

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[[Image:Balloonconcept.jpg]]
[[Image:Balloonconcept.jpg]]
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When light shines on E. coli,,,
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The light in the morning leaking from a gap between the curtains... this morning lights calls and awakes the E. coli.
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'''E. coli become bigger and bigger like BALLOON !!!'''  
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Suprise!!
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 +
The E. coli become bigger and bigger like BALLOON!!!  
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'''→World first bacteria that swells by lights.!!!'''
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 +
 
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=Design=
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to stop the e.coli by light, we needed a part that stop the e.coli, and combine this with a light receptor that UCSF made it last year.
 +
 
 +
there was some ways to stop the e.coli running:
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#making a chemaera with chemotaxis receptor and light sensing receptor
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#stop the motor protein's expression(motB protein: from last year's parts)
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#controling chemotaxis
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etc
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we chose to control chemotaxis.
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We've found out, from the paper(..see refrences), the chemoreceptor that fix the e.coli tumble.
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it is a fragment of the chemoreceptor "Tsr" (a serin receptor)from aa 290 to 551. we call this tsr-cw(or tsr290).
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<br />
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[[Image:pathway.jpg|pathway]]

Revision as of 07:49, 30 October 2006

Concept

Balloonconcept.jpg

The light in the morning leaking from a gap between the curtains... this morning lights calls and awakes the E. coli.

Suprise!!

The E. coli become bigger and bigger like BALLOON!!!

→World first bacteria that swells by lights.!!!


Design

to stop the e.coli by light, we needed a part that stop the e.coli, and combine this with a light receptor that UCSF made it last year.

there was some ways to stop the e.coli running:

  1. making a chemaera with chemotaxis receptor and light sensing receptor
  2. stop the motor protein's expression(motB protein: from last year's parts)
  3. controling chemotaxis

etc

we chose to control chemotaxis. We've found out, from the paper(..see refrences), the chemoreceptor that fix the e.coli tumble. it is a fragment of the chemoreceptor "Tsr" (a serin receptor)from aa 290 to 551. we call this tsr-cw(or tsr290).


pathway


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