Biobricks

(Difference between revisions)

Revision as of 12:05, 29 August 2006

Parts	Function	Status
E. coli ArsR to LacZ	pH lowering output in response to raised arsenate concentration	Not Working
B. subtilis ArsR to LacZ	As Above	Doing again with correct lacZ
B. subtilis ArsR to lambda cI repressor	To repress the hybrid promoter in response to lower levels of arsenate	Done but untested
GFP to Terminator	Testing ArsR function if can't get pH response	Done but not ligated to either ArsR

Biobrick	Construction	Ligations	Testing	Sequencing	Use
E. coli ArsR	Complete	To LacZ	Not Working	Not Yet	Detection of Arsenic
B. subtilis ArsR	Complete	To LacZ	Not Tested	Not Yet	Detection of Arsenic
LacZ	Complete	To ArsR and Terminator	Tests Underway	Not Yet	Lowering pH/Reporting
Bacillus Urease	Site specific mutagenesis required	To ligate to hybrid promoter	N/A	Not Yet	Raising pH
lambda cI/lacI Promoter	Underway	To ligate to urease	N/A	Not yet	Repressed by lambda cI and lacI in absence of lactose

Biobrick	Number and Location	Ligations	Testing	Use
Terminator	BBa_B0015, 1I	To LacZ, plasmid used as vector for most ligations	N/A	Terminating
RBS	BBa_B0034, 3O	To ArsR from E. coli	N/A	Ribosome binding site
lambda cI	BBa_R0051, 9C	Not yet	Not yet	Repressing hybrid promoter

All biobricks have to have the following:

Prefix

(5’) cctttctagag (3’)

Suffix

(5’) tactagtagcggccgctgcagcctt (3’)

[http://2006.igem.org/University_of_Edinburgh_2006 Main page]

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 | B. subtilis ArsR to lambda cI repressor || To repress the hybrid promoter in response to lower levels of arsenate || Done but untested
+|-
+| GFP to Terminator || Testing ArsR function if can't get pH response || Done but not ligated to either ArsR
 |-
 |}