ETH 2006 Half adder

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(reverted to state of July 31 when we decided for the half adder based on this information. The documentation of our further work was moved to the progress report on the front page.)
(Main Goal:)
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====Main Goal:====
====Main Goal:====
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*Write something with a chemical on a petri plate (like '''ETH''' for example)
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# Write something with a chemical on a petri plate (like '''ETH''' for example)
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*Let Bacteria grow uniformly on the plate
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# Let Bacteria grow uniformly on the plate
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*Expose the plate to a picture (black and white) of the same pattern  
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# Expose the plate to a picture (black and white) of the same pattern  
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*Result:
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# Result:
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**Bacteria gets green when pattern on the plate and picture match (light and chemical)
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#*Bacteria gets green when pattern on the plate and picture match (light and chemical)
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**Bacteria does not express fluorescent protein when pattern on the plate and picture match (no light and no chemical)
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#*Bacteria does not express fluorescent protein when pattern on the plate and picture match (no light and no chemical)
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**Bacteria gets red when pattern on the plate and picture do not match
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#*Bacteria gets red when pattern on the plate and picture do not match
====Implementation from the ingenieur point of view====
====Implementation from the ingenieur point of view====

Revision as of 15:30, 11 October 2006

back to → ETH 2006 Main PageMeat Monitor ideaother project ideas

Contents

Half-adder or Full-adder

An implementation of a half-adder or full-adder: 1-bit adder with carry

  • [http://en.wikipedia.org/wiki/Full_adder half/full adder in wikipedia]

Idea: Pattern recognition

→ Illustration of the concepts:

Main Goal:

  1. Write something with a chemical on a petri plate (like ETH for example)
  2. Let Bacteria grow uniformly on the plate
  3. Expose the plate to a picture (black and white) of the same pattern
  4. Result:
    • Bacteria gets green when pattern on the plate and picture match (light and chemical)
    • Bacteria does not express fluorescent protein when pattern on the plate and picture match (no light and no chemical)
    • Bacteria gets red when pattern on the plate and picture do not match

Implementation from the ingenieur point of view

  • Half Adder
  • = opportunity to build an AND and an XOR Gate

Implementation from the biologist point of view

  • AND Gate: presence of chemical induces the production of an inactive protein, which is activated by a second protein (produced when light is present): the first protein is then activating GFP production
  • XOR Gate: I think Marko had a good idea about that (I didn't really got it)
  • a light sensible promoter (there are some, see [http://partsregistry.org/Featured_Parts:Light_Sensor here])
  • a chemical sensible promoter (chemical to be defined)
  • we would need to give a kind of first signal to the system (to don't have light reactions before a certain moment, otherwise we have to work in a dark room...), an activation signal after the bacterias have grown on the plate

Pro's & Con's

Pro's:

  • meaningful from engineering point
  • valuable parts for synthetic biology
  • stepwise proceeding applicable (→ intermediate and partial results!)
  • experiments visually attractive
  • probably simple enough

Con's:

  • cheap copy of “bio-film” project ([http://partsregistry.org/cgi/htdocs/SBC04/index.cgi iGEM 2004])
  • sensational experiments, have little in common with half adder
  • maybe that a big vision is lacking
  • general/practical benefit? (counter-argument: is this required?)
  • too simple?
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