Talk:UT Austin

From 2006.igem.org

Revision as of 22:51, 7 July 2005

• Testing I15030 [http://rosalind.csail.mit.edu/r/parts/partsdb/view.cgi?part_id=5414], the HSL bomb (07.07.05)

• Aaron

Can work up (and show rachel, laura and jeff) how to do liquid phase LacZ assays. Also can work up the solid phase hsl bomb experiments. We will start these using minimal media A (unbuffered) at 37C as in basu et al., nature, 2005. they solidify their media with 1.5% agarose. We can use seakem LE agarose (the cheap stuff) for this purpose to begin with.

The plates should be Amp/Kan because I15030 is in psb3k3 and all of the reporters are in psb1a2. also, we should probably use S-gal in the plates.

• Rachel

Building R0065.B0034.LacZalpha We need to submit the correct LacZalpha sequence into the registry, and give it a biobricked name. Then we need to submit R0065.B0034 as a part, and R0065.B0034.LacZalpha as a part.

We will hold off on making F2620.E0430, because that is already constructed at MIT (bba_I13273 [http://rosalind.csail.mit.edu/r/parts/partsdb/view.cgi?part_id=5121]) and we will be getting it in a week or two. Moreover, I6042 will function just like I13273 if it is provided with a source of LuxR.

• Laura

Prep out pSB1A2.I6042 [http://rosalind.csail.mit.edu/r/parts/partsdb/view.cgi?part_id=4471] (MIT 4.04) and cotransform into omnimax cells with psb3k3.I15030 [http://rosalind.csail.mit.edu/r/parts/partsdb/view.cgi?part_id=5414]. this will be a yfp tester construct for the hsl bomb. Also test pSB1A2.R0065.B0034.LacZ made by JT last year as a reporter for the edge detector. This construct has 6 mutations, so we need to see if it still produces a functional LacZ protein

• Dan

Dan is currently deriving a mathematical model to analyze the light wires design.