ETH2006 iptg
From 2006.igem.org
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[[Image:ETH_IPTG_sensing_parts.png|center|600px|complete system based on alternative 1]] | [[Image:ETH_IPTG_sensing_parts.png|center|600px|complete system based on alternative 1]] | ||
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| + | === modeling === | ||
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| + | [[ETH_Sim_Mod_IPTG|simulation results]] | ||
=== assembly procedure === | === assembly procedure === | ||
Revision as of 13:49, 26 October 2006
Contents |
chemical sensing device
The chemical sensing device's PoPs output activity should be a monotonic function of the concentration of a certain substance in the cell's environment.
implementation alternatives
- Lactate lacI represses, IPTG induces ([http://partsregistry.org/Part:BBa_R0011 BBa_R0011] or [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] )
- Tetracycline, TetR inhibitor, Tet inducer by inhibiting TetR (or aTc, it's analog) ([http://partsregistry.org/Part:BBa_R0040 BBa_R0040])
- combination thereof ([http://partsregistry.org/Part:BBa_I13614 BBa_I13614] / [http://partsregistry.org/Part:BBa_I13617 BBa_13617] / [http://partsregistry.org/Part:BBa_I13623 BBa_I13623] / [http://partsregistry.org/Part:BBa_I13624 BBa_I13624] / [http://partsregistry.org/Part:BBa_I13627 BBa_I13627] / [http://partsregistry.org/Part:BBa_I13637 BBa_I13637] / [http://partsregistry.org/Part:BBa_I13653 BBa_I13653])
- simple sugar Arabinose ([http://partsregistry.org/Part:BBa_R0080 BBa_R0080])
I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. since the inducers are water-soluble we would have to fix the chemicals onto the petri dish.
modeling
assembly procedure
where we got it and link to theyer documentation
Progress:
- 2006/10/03
- Made LB-Agar plates with antibiotics
- 2006/10/04
- Transformed cells, plated them
- 2006/10/05
- Found plates empty after 18h on the table, put into incubator at 37°C
- 2006/10/06
- picked cultures onto fresh plates
