The 1st South American team synthetic machine
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| - | + | [[pSB1A3]]-[[PFe]]-[[mRFP]] ([[PI + PII ]]+ [[BBa_J04450 ]] | |
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| + | == SUMMARY == | ||
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| - | Escherichia coli strain ( DH5α ) was transformed with vector pSB1A3 containing promoters PI and PII from acidothiobacilus rus operon fused with device BBa_J04450, wich contains a monomeric red | + | Escherichia coli strain ( DH5α ) was transformed with vector [[pSB1A3]] containing promoters PI and PII from acidothiobacilus rus operon fused with device [[BBa_J04450 ]] , wich contains a monomeric red fluorescent protein [[mRFP]], respectively and to a fluorescent protein coding region of 2545 bp from 5´-3´. The pSB1A3- P Fe- mRFP was standardized for its specific sensoribility response to iron ions, in order to detect iron contamination and corrosion. Iron (II) was used at different concentrations (0, 1, 50 and 100 ppm). The pSB1A3-Fe was grown under presence and absence of UV, oxygen, and different levels of pH. The biosensoribility was determined by the response of the pSB1A2-UV-Fe to the different concentrations of iron. This response was measured by, presence of fluorescence, DNA concentration, bacterial growth and changes on growth medium . These parameters were related to sensoribility of the 1st South American team synthetic machine. |
Revision as of 06:52, 29 October 2006
pSB1A3-PFe-mRFP (PI + PII + BBa_J04450
SUMMARY
Escherichia coli strain ( DH5α ) was transformed with vector pSB1A3 containing promoters PI and PII from acidothiobacilus rus operon fused with device BBa_J04450 , wich contains a monomeric red fluorescent protein mRFP, respectively and to a fluorescent protein coding region of 2545 bp from 5´-3´. The pSB1A3- P Fe- mRFP was standardized for its specific sensoribility response to iron ions, in order to detect iron contamination and corrosion. Iron (II) was used at different concentrations (0, 1, 50 and 100 ppm). The pSB1A3-Fe was grown under presence and absence of UV, oxygen, and different levels of pH. The biosensoribility was determined by the response of the pSB1A2-UV-Fe to the different concentrations of iron. This response was measured by, presence of fluorescence, DNA concentration, bacterial growth and changes on growth medium . These parameters were related to sensoribility of the 1st South American team synthetic machine.
