The 1st South American team synthetic machine

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Escherichia coli strain ( DH5α ) was transformed with vector pSB1A3[http://partsregistry.org/Part:pSB1A2] containing promoters PI and PII from acidothiobacilus rus operon [http://mic.sgmjournals.org/cgi/content/abstract/150/7/2113] fused with  device [[BBa_J04450 ]] respectively , wich at 2545 bp (+) codes a monomeric red  fluorescent protein [[mRFP]]. The pSB1A3-PFe- mRFP was standardized for its specific sensoribility response to iron ions, in order to detect iron contamination and corrosion. Iron (II) was used at different concentrations (0, 1, 50 and 100 ppm). The pSB1A3-Fe was grown under presence and absence of UV, oxygen, and different levels of pH. The biosensorbility was determined by the response of the pSB1A2-UV-Fe to the different concentrations of iron. This response was measured by presence/absence of fluorescence, meanwhile,  foton-fenton interaction was measured by  DNA concentration, bacterial growth and changes on growth medium (like pH, mV) . once performed,  parameters were related to sensorbility of the 1st South American team  synthetic  machine
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Escherichia coli strain ( DH5α ) was transformed with vector pSB1A3[http://partsregistry.org/Part:pSB1A2] containing promoters PI and PII from acidothiobacilus rus operon [http://mic.sgmjournals.org/cgi/content/abstract/150/7/2113] fused with  device BBa_J04450 [http://partsregistry.org/Part:BBa_J04450] respectively , wich at 2545 bp (+) codes a monomeric red  fluorescent protein [[mRFP]]. The pSB1A3-PFe- mRFP was standardized for its specific sensoribility response to iron ions, in order to detect iron contamination and corrosion. Iron (II) was used at different concentrations (0, 1, 50 and 100 ppm). The pSB1A3-Fe was grown under presence and absence of UV, oxygen, and different levels of pH. The biosensorbility was determined by the response of the pSB1A2-UV-Fe to the different concentrations of iron. This response was measured by presence/absence of fluorescence, meanwhile,  foton-fenton interaction was measured by  DNA concentration, bacterial growth and changes on growth medium (like pH, mV) . once performed,  parameters were related to sensorbility of the 1st South American team  synthetic  machine

Revision as of 01:36, 31 October 2006

PrFe-mRFP1



SUMMARY

Escherichia coli strain ( DH5α ) was transformed with vector pSB1A3[http://partsregistry.org/Part:pSB1A2] containing promoters PI and PII from acidothiobacilus rus operon [http://mic.sgmjournals.org/cgi/content/abstract/150/7/2113] fused with device BBa_J04450 [http://partsregistry.org/Part:BBa_J04450] respectively , wich at 2545 bp (+) codes a monomeric red fluorescent protein mRFP. The pSB1A3-PFe- mRFP was standardized for its specific sensoribility response to iron ions, in order to detect iron contamination and corrosion. Iron (II) was used at different concentrations (0, 1, 50 and 100 ppm). The pSB1A3-Fe was grown under presence and absence of UV, oxygen, and different levels of pH. The biosensorbility was determined by the response of the pSB1A2-UV-Fe to the different concentrations of iron. This response was measured by presence/absence of fluorescence, meanwhile, foton-fenton interaction was measured by DNA concentration, bacterial growth and changes on growth medium (like pH, mV) . once performed, parameters were related to sensorbility of the 1st South American team synthetic machine


OBJECTIVES

Assemble a machine for iron detection to elucidate relation Photon (UV)-Phenton(Fe (II)) as a source of energy in extreme conditions such as Mars (ie. absence of oxigen, permanet presence of UV ligth irradiation and diferent concentratios of iron(II) as unic electron aceptor).

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