Sorting Bacteria

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=The Aggregation approach=
=The Aggregation approach=
==Basic Idea==
==Basic Idea==
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The idea would be to have two types of E. coli :  
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The idea would be to have two types of E. coli : Type G would express GFP (they look green), other type R would express RFP (they look red)
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One type would express GFP (they look green)
 
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The other type would express RFP (they look red)
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The starting material is an homogeneous mixture of red and green, i.e. of G- and R-type bacteria.
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The starting material is an unorganized mixture of red and green bacteria.  
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The G-type bacteria would start, under induction, to express an aggregation factor, e.g. Antigen 43 (Ag43) working on stable media culture. Thus, the G-type bacteria would start to gather forming green dots on the plate.  
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The R-type bacteria express their own aggregation factor (Ag43), but with a certain delay and thus leaving time to the G-type bacteria to sufficiently cluster first.
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The GFP expressing bacteria would start, under induction, to express Antigen 43 (Ag43)
 
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which is an aggregation factor working on stable media culture.
 
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The "green" bacteria would start to gather making green dots on the plates.
 
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The "red" bacteria express, with a certain delay(long enough to let the green ones to
 
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cluster first), the aggregation factor (Ag43).
 
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This would produce a sorted phenotype (green bacteria surrounded by red bacteria)
 
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This would produce a sorting behavior, i.e. green bacteria surrounded by red bacteria the latter probably forming rings.
==possible Extensions==
==possible Extensions==

Revision as of 12:35, 4 August 2005

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Contents

Intro

Emergent sorting.

Principle

Two populations of bacteria with different clustering behavior and colors.

The Aggregation approach

Basic Idea

The idea would be to have two types of E. coli : Type G would express GFP (they look green), other type R would express RFP (they look red)


The starting material is an homogeneous mixture of red and green, i.e. of G- and R-type bacteria.


The G-type bacteria would start, under induction, to express an aggregation factor, e.g. Antigen 43 (Ag43) working on stable media culture. Thus, the G-type bacteria would start to gather forming green dots on the plate.

The R-type bacteria express their own aggregation factor (Ag43), but with a certain delay and thus leaving time to the G-type bacteria to sufficiently cluster first.


This would produce a sorting behavior, i.e. green bacteria surrounded by red bacteria the latter probably forming rings.

possible Extensions

Discussion

>> for comments, questions and temporary remarks go to the Talk:Sorting_Bacteria

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