Sorting Bacteria
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The idea would be to have two types of E. coli : Type G would express GFP (they look green), other type R would express RFP (they look red) | The idea would be to have two types of E. coli : Type G would express GFP (they look green), other type R would express RFP (they look red) | ||
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The starting material is an homogeneous mixture of red and green, i.e. of G- and R-type bacteria. | The starting material is an homogeneous mixture of red and green, i.e. of G- and R-type bacteria. | ||
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The G-type bacteria would start, under induction, to express an aggregation factor, e.g. Antigen 43 (Ag43) working on stable media culture. Thus, the G-type bacteria would start to gather forming green dots on the plate. | The G-type bacteria would start, under induction, to express an aggregation factor, e.g. Antigen 43 (Ag43) working on stable media culture. Thus, the G-type bacteria would start to gather forming green dots on the plate. | ||
The R-type bacteria express their own aggregation factor (Ag43), but with a certain delay and thus leaving time to the G-type bacteria to sufficiently cluster first. | The R-type bacteria express their own aggregation factor (Ag43), but with a certain delay and thus leaving time to the G-type bacteria to sufficiently cluster first. | ||
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This would produce a sorting behavior, i.e. green bacteria surrounded by red bacteria the latter probably forming rings. | This would produce a sorting behavior, i.e. green bacteria surrounded by red bacteria the latter probably forming rings. | ||
Revision as of 12:35, 4 August 2005
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Contents |
Intro
Emergent sorting.
Principle
Two populations of bacteria with different clustering behavior and colors.
The Aggregation approach
Basic Idea
The idea would be to have two types of E. coli : Type G would express GFP (they look green), other type R would express RFP (they look red)
The starting material is an homogeneous mixture of red and green, i.e. of G- and R-type bacteria.
The G-type bacteria would start, under induction, to express an aggregation factor, e.g. Antigen 43 (Ag43) working on stable media culture. Thus, the G-type bacteria would start to gather forming green dots on the plate.
The R-type bacteria express their own aggregation factor (Ag43), but with a certain delay and thus leaving time to the G-type bacteria to sufficiently cluster first.
This would produce a sorting behavior, i.e. green bacteria surrounded by red bacteria the latter probably forming rings.
possible Extensions
Discussion
>> for comments, questions and temporary remarks go to the Talk:Sorting_Bacteria
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