Biobrick delivery
From 2006.igem.org
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'''What do we do with this plate once we have received it?''' | '''What do we do with this plate once we have received it?''' | ||
<br>The DNA in each well needs to be resuspended. To do this you must: | <br>The DNA in each well needs to be resuspended. To do this you must: | ||
| - | :# | + | :# Puncture a hole through the foil with a pipette tip (be sure you have the correct well) |
:# Add X ul of TE Buffer (Tris-HCl?) | :# Add X ul of TE Buffer (Tris-HCl?) | ||
:# Take 1ul DNA and transform into your favorite competent cells, plate out on a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies. | :# Take 1ul DNA and transform into your favorite competent cells, plate out on a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies. | ||
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'''What is that red (or orange, green, yellow) stuff at the bottom of the wells?''' | '''What is that red (or orange, green, yellow) stuff at the bottom of the wells?''' | ||
| - | <br>What you see at the bottom of the wells is your dried DNA (see picture above). It is color-coded according to our [http://partsregistry.org/Help:How_to_make_agar standard color key]. | + | <br>What you see at the bottom of the wells is your dried DNA (see picture above). It is color-coded with food coloring according to our [http://partsregistry.org/Help:How_to_make_agar standard color key]. |
'''How do we know what the DNA is in each well?''' | '''How do we know what the DNA is in each well?''' | ||
<br>By the time you receive your dried DNA plates, there will be a page on the [http://partsregistry.org/Main_Page Registry] where you can look up which parts are in which wells. | <br>By the time you receive your dried DNA plates, there will be a page on the [http://partsregistry.org/Main_Page Registry] where you can look up which parts are in which wells. | ||
Revision as of 12:40, 21 April 2006
One of the resources that MIT provides teams competing in iGEM is a copy of parts that have been submitted to the Registry. These parts also include those that were submitted as a result of previous iGEM competitions (a big reason why we want you to keep on top of [http://partsregistry.org/Help:How_to_send_parts sending] us your parts as you create them!). For iGEM 2006, the Registry will send out parts in the form of dried DNA.
Sometime in the next several weeks you will receive a package in the mail containing a plate that looks like this:
What do we do with this plate once we have received it?
The DNA in each well needs to be resuspended. To do this you must:
- Puncture a hole through the foil with a pipette tip (be sure you have the correct well)
- Add X ul of TE Buffer (Tris-HCl?)
- Take 1ul DNA and transform into your favorite competent cells, plate out on a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.
- Pick a single colony and inoculate some broth (with the correct antibiotic) and grow ~18 hours.
- Use the resulting culture to miniprep AND make your own glycerol stock (for further instruction on making a glycerol see [http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria this page]).
This DNA is your own collection of parts that you can use to create your own parts. By making your own glycerols you now have your own stock to go back to and grow/prep whenever you need that particular part.
What is that red (or orange, green, yellow) stuff at the bottom of the wells?
What you see at the bottom of the wells is your dried DNA (see picture above). It is color-coded with food coloring according to our [http://partsregistry.org/Help:How_to_make_agar standard color key].
How do we know what the DNA is in each well?
By the time you receive your dried DNA plates, there will be a page on the [http://partsregistry.org/Main_Page Registry] where you can look up which parts are in which wells.
