McGill University 2006
From 2006.igem.org
(→Parts shopping list) |
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'''All these bricks express YFP and downregulate it in response to a signal''' | '''All these bricks express YFP and downregulate it in response to a signal''' | ||
| - | + | YELLOW | |
| + | |||
| + | ARABINOSE RESPONSE. | ||
BBa_E0610: Arabinose turns on lac repressor which in turn shuts down Yellow fluorescent protein. | BBa_E0610: Arabinose turns on lac repressor which in turn shuts down Yellow fluorescent protein. | ||
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BBa_E0603: Like BBa_E0602 but baseline expression of YFP is higher. Not listed as available | BBa_E0603: Like BBa_E0602 but baseline expression of YFP is higher. Not listed as available | ||
| + | |||
| + | BBa_E0604: Arabinose turns on destabilized 434 cl epressor which in turn shuts down destabilized (slow) Yellow fluorescent protein. Not listed as available | ||
| + | |||
| + | BBa_E0614: Like BBa_E0604 but YFP is not destabilized (hangs around forever). Not listed as available | ||
| + | |||
| + | BBa_E0605: Arabinose turns on destabilized p22 epressor which in turn shuts down destabilized (slow) Yellow fluorescent protein. Not listed as available | ||
| + | |||
| + | BBa_E0615: Like BBa_E0605, but YFP is not destabilized, hangs around forever. | ||
| + | |||
| + | |||
| + | CYAN | ||
| + | |||
| + | TET response | ||
| + | |||
| + | BBa_I0401: Expresses CFP and lux from the Tet-OFF promoter. Neither is destabilized. Not listed as available | ||
| + | |||
| + | BBa_I0402: Like BBa_I0401 but lux is destabilized (LVA, slow) | ||
| + | |||
| + | BBa_I0404: Produces rhlR and CFP, downregulated by Tet. rhlR binds with N-butyryl-HSL, regulates transcription. rhlR but not CFP is destabilized. | ||
| + | |||
| + | Arabinose response: | ||
| + | |||
| + | BBa_I0403 Expresses CFP and aiiA from an arabinose promoter. aiiA degrades N-acyl homoserine lactones (quorum sensing autoinducers). aiiA but not CFP is destabilized (LVA, slow). | ||
=Introduction= Our brainstorming meeting on 3-16 gave us several preliminary ideas which we will explore via modeling and research | =Introduction= Our brainstorming meeting on 3-16 gave us several preliminary ideas which we will explore via modeling and research | ||
Revision as of 21:00, 25 April 2006
Contents |
Name: Fousion
News
- 2006-04-14 Discussion page created
- 2006-03-16 First kick-off meeting and team selection
Organisation
People
Students
Supervisors
| Jay Nadeau |
Timeline
Tasks
Parts shopping list
Composite bricks - these are the best to use as less cloning is required.
All these bricks express YFP and downregulate it in response to a signal
YELLOW
ARABINOSE RESPONSE.
BBa_E0610: Arabinose turns on lac repressor which in turn shuts down Yellow fluorescent protein.
BBa_E0600: like BBa_E0610 but YFP is destabilized - slow degradation. Not listed as available.
BBa_E0611: Arabinose turns on destabilized TetR repressor which shuts down yellow fluorescent protein. Addition of Tetracyline blocks TetR repressor allowing yellow fluorescent protein to be turned back on.
BBa_E0601: Like BBa_E0611 but YFP is destabilized - slow degradation. Not listed as available
BBa_E0613: Arabinose turns on destabilized lambda repressor which shuts down yellow fluorescent protein.
BBa_E0602: Like BBa_E0613 but YFP is destabilized - slow degradation. Not listed as available
BBa_E0603: Like BBa_E0602 but baseline expression of YFP is higher. Not listed as available
BBa_E0604: Arabinose turns on destabilized 434 cl epressor which in turn shuts down destabilized (slow) Yellow fluorescent protein. Not listed as available
BBa_E0614: Like BBa_E0604 but YFP is not destabilized (hangs around forever). Not listed as available
BBa_E0605: Arabinose turns on destabilized p22 epressor which in turn shuts down destabilized (slow) Yellow fluorescent protein. Not listed as available
BBa_E0615: Like BBa_E0605, but YFP is not destabilized, hangs around forever.
CYAN
TET response
BBa_I0401: Expresses CFP and lux from the Tet-OFF promoter. Neither is destabilized. Not listed as available
BBa_I0402: Like BBa_I0401 but lux is destabilized (LVA, slow)
BBa_I0404: Produces rhlR and CFP, downregulated by Tet. rhlR binds with N-butyryl-HSL, regulates transcription. rhlR but not CFP is destabilized.
Arabinose response:
BBa_I0403 Expresses CFP and aiiA from an arabinose promoter. aiiA degrades N-acyl homoserine lactones (quorum sensing autoinducers). aiiA but not CFP is destabilized (LVA, slow).
=Introduction= Our brainstorming meeting on 3-16 gave us several preliminary ideas which we will explore via modeling and research
