Answer411
From 2006.igem.org
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When core sequences are mismatched on the HIN-H107Y mutant, ligation is prevented but synapsis and cleavage is allowed. After recombination, the sites with different core sequences create DNA knots due to "inability to base-pair at crossover site after single exchange of DNA. A second exchange that restores the parental sequence of the recombination sties, but ties a knot in the plasmid DNA, is required for ligation." This would be a possible way to allow for a series of flipping sites to be side by side and keep them from interacting with each other. This question is still unanswered, but interesting info found along the way. | When core sequences are mismatched on the HIN-H107Y mutant, ligation is prevented but synapsis and cleavage is allowed. After recombination, the sites with different core sequences create DNA knots due to "inability to base-pair at crossover site after single exchange of DNA. A second exchange that restores the parental sequence of the recombination sties, but ties a knot in the plasmid DNA, is required for ligation." This would be a possible way to allow for a series of flipping sites to be side by side and keep them from interacting with each other. This question is still unanswered, but interesting info found along the way. | ||
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| + | http://www.bio.davidson.edu/courses/synthetic/papers/Sanders_04.pdf | ||
Latest revision as of 16:35, 30 May 2006
When core sequences are mismatched on the HIN-H107Y mutant, ligation is prevented but synapsis and cleavage is allowed. After recombination, the sites with different core sequences create DNA knots due to "inability to base-pair at crossover site after single exchange of DNA. A second exchange that restores the parental sequence of the recombination sties, but ties a knot in the plasmid DNA, is required for ligation." This would be a possible way to allow for a series of flipping sites to be side by side and keep them from interacting with each other. This question is still unanswered, but interesting info found along the way.
http://www.bio.davidson.edu/courses/synthetic/papers/Sanders_04.pdf
