Davidson Parts
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'''''Recombinational Enhancer''''' | '''''Recombinational Enhancer''''' | ||
| - | These are the | + | These are the oligos that we ordered to make the recombinational enhancer. |
AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT | AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT | ||
Revision as of 02:53, 7 June 2006
Hin gene
Primers for Hin gene the 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in bold.
Forward primer
5’ gcattctagaatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttacCagtgcaaattg 3’
Reverse primer
5’ atgcctgcaggcggccgcactagtttaattcattcgtttttttatac 3’
Recombinational Enhancer
These are the oligos that we ordered to make the recombinational enhancer.
AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT
CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC
ATTTTACTAGTTGCGGCCGCCTGCA
GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA
AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG
