Plasmids and Bacteria
From 2006.igem.org
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These sequences will be transformed into RFP and possibly GFP plasmids | These sequences will be transformed into RFP and possibly GFP plasmids | ||
| + | *Doing Minipreps to isolate the RFP and GFP plasmids | ||
Revision as of 17:15, 8 June 2006
- pSB1A3
- amp resistant
- copy number:100-300
- size:2157 empty
- pSB1AC3
- part-BBAp1000
- copy number:100-3000
- amp and CmR resistant
- contains device that provides CmR
- size:3844 ( with device) 3005 (without device)
- pSB1AK3
- part BBa_|13535
- copy number:100-300
- amp and kan resistance
- contains a device for screening test
- size: 3457 (with device) 3189 (without device)
- Transform bacteria We are using Salmonella typhimurium for the Hin protein as it was the referenced species in all of the literature.
- Create RE biobrick (completed)
- Isolating genomic DNA from Salmonella typhimurium (completed)
- Ordered oligos for recombinational enhancer and primers for the hin gene
These sequences will be transformed into RFP and possibly GFP plasmids
- Doing Minipreps to isolate the RFP and GFP plasmids
