Hin gene: Part BBa J31000
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'''''Hin gene: Part BBa_J31000''''' | '''''Hin gene: Part BBa_J31000''''' | ||
| - | Primers for Hin gene the 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in,<font color='blue'> | + | Primers for Hin gene the 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in,<font color='blue'>blue</font color>. |
'''Forward primer''' | '''Forward primer''' | ||
| - | <font face="courier new">5’ GCATTCTAGAATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTAC<font color='blue'> | + | '''<font face="courier new">5’ GCATTCTAGAATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTAC<font color='blue'>C</font color>AGTGCAAATTG 3’</font face>''' |
'''Reverse primer''' | '''Reverse primer''' | ||
| - | <font face="courier new">5’ ATGCCTGCAGGCGGCCGCACTAGTTTAATTCATTCGTTTTTTTATAC 3’</font face> | + | '''<font face="courier new">5’ ATGCCTGCAGGCGGCCGCACTAGTTTAATTCATTCGTTTTTTTATAC 3’</font face>''' |
Revision as of 18:43, 12 June 2006
Hin gene: Part BBa_J31000
Primers for Hin gene the 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in,blue.
Forward primer
5’ GCATTCTAGAATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTACCAGTGCAAATTG 3’
Reverse primer
5’ ATGCCTGCAGGCGGCCGCACTAGTTTAATTCATTCGTTTTTTTATAC 3’
