Cloning hix fragments
From 2006.igem.org
(Difference between revisions)
Revision as of 15:04, 18 June 2006
Set up four ligations as follows, with vector only and vector with each of the three annealed hix oligos from the right freezer:
4 ul of the purified EcorI/PstI digested vector from pTet-GFP 2 ul annealed hixL, hix R, hixC, or H2O 3 ul H2O 10 ul 2X Quick ligase buffer 1 ul Quick T4 ligase
room temp 5 min
