Plasmid Prep
From 2006.igem.org
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| - | Using overnight culture of plasmid I13522 (pTet-GFP), do plasmid prep on | + | Using pellets from overnight culture of plasmid I13522 (pTet-GFP), do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. |
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows: | Combine the eluted plasmid from all the preps into one tube and measure the yield as follows: | ||
Revision as of 15:15, 18 June 2006
Using pellets from overnight culture of plasmid I13522 (pTet-GFP), do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge.
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows:
- turn on UV spec and blank with H2O
- measure A260 of 4 ul plasmid + 76 ul H2O
- this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul
