Plasmid Prep
From 2006.igem.org
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| - | Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP). | + | Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP). (Done by Brad and Eric -- Monday morning. Followed plasmid prep protocol.) |
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows: | Combine the eluted plasmid from all the preps into one tube and measure the yield as follows: | ||
Revision as of 15:07, 19 June 2006
Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP). (Done by Brad and Eric -- Monday morning. Followed plasmid prep protocol.)
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows:
- turn on UV spec and blank with H2O
- measure A260 of 4 ul plasmid + 76 ul H2O
- this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul
