Plasmid Prep
From 2006.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP). | + | Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP). |
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows: | Combine the eluted plasmid from all the preps into one tube and measure the yield as follows: | ||
| Line 7: | Line 7: | ||
# this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul | # this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul | ||
| - | Plasmid prep was done by Brad and Eric and I ( | + | Plasmid prep was done by Brad and Eric (Followed plasmid prep protocol), and I (Kelly) jumped in to help with the A260 readings. We obtained 71 ng/uL. |
Latest revision as of 18:55, 19 June 2006
Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP).
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows:
- turn on UV spec and blank with H2O
- measure A260 of 4 ul plasmid + 76 ul H2O
- this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul
Plasmid prep was done by Brad and Eric (Followed plasmid prep protocol), and I (Kelly) jumped in to help with the A260 readings. We obtained 71 ng/uL.
