Things That we Have Done

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Cloning

We have been able to successfully clone the following antibiotic pancakes

  • Hin
  • Hin with LVA degredation tag
  • Cmr- forward
  • Kan-forward
  • Tet-forward
  • Tet-backwards

RE and Hix

We have been able to clone RE into cells. The RE that we are using has two point mutations but they should not compromise the activity of the Fis-binding sites please see the page for part [http://partsregistry.org/Part:BBa_J3101| Part BBa_J3101] for further details.

  • As per the [http://2006.igem.org/Davidson_Assembly_Plan| assembly plan] we will be attaching the RE downstream of the double terminator [http://partsregistry.org/Part:BBa_B0015| BBa_B0015].

We also have two cultures with the correct sequences for Hix C.

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