Things That we Have Done
From 2006.igem.org
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=RE and Hix= | =RE and Hix= | ||
We have been able to clone RE into cells. The RE that we are using has two point mutations but they should not compromise the activity of the Fis-binding sites please see the page for part [http://partsregistry.org/Part:BBa_J3101| Part BBa_J3101] for further details. | We have been able to clone RE into cells. The RE that we are using has two point mutations but they should not compromise the activity of the Fis-binding sites please see the page for part [http://partsregistry.org/Part:BBa_J3101| Part BBa_J3101] for further details. | ||
| - | * As per the [http://2006.igem.org/Davidson_Assembly_Plan| assembly plan] we will be attaching the RE downstream of the double terminator [http://partsregistry.org/Part: | + | * As per the [http://2006.igem.org/Davidson_Assembly_Plan| assembly plan] we will be attaching the RE downstream of the double terminator [http://partsregistry.org/Part:BBa_J3101| BBa_J3101] |
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We also have two cultures with the correct sequences for Hix C. | We also have two cultures with the correct sequences for Hix C. | ||
Revision as of 16:08, 12 July 2006
Cloning
We have been able to successfully clone the following antibiotic pancakes
- Hin
- Hin with LVA degredation tag
- Cmr- forward
- Kan-forward
- Tet-forward
- Tet-backwards
RE and Hix
We have been able to clone RE into cells. The RE that we are using has two point mutations but they should not compromise the activity of the Fis-binding sites please see the page for part [http://partsregistry.org/Part:BBa_J3101| Part BBa_J3101] for further details.
- As per the [http://2006.igem.org/Davidson_Assembly_Plan| assembly plan] we will be attaching the RE downstream of the double terminator [http://partsregistry.org/Part:BBa_J3101| BBa_J3101]
We also have two cultures with the correct sequences for Hix C.
