Davidson Assembly Plan
From 2006.igem.org
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Upon receipt of the Hin Expression Unit (no Hix) from Western, Davidson will assemble the following and complete the assembly of Construct 2 and Construct 2 – Control. | Upon receipt of the Hin Expression Unit (no Hix) from Western, Davidson will assemble the following and complete the assembly of Construct 2 and Construct 2 – Control. | ||
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Revision as of 21:59, 12 July 2006
Parts List
pLac promoter -- BBa_R0010 DNA-1 7K
pAra promoter -- BBa_I13453 DNA-2 13D
RBS -- BBa_B0030 DNA-1 3G
HixC – BBa_B?????
double forward terminator -- BBa_B0015 DNA-1 1I
double backward terminator – BBa_B0025 DNA-1 3E
KanR – BBa_B?????
Recombination enhancer -- BBa_J3101 (Davidson)
Hin -- BBa_J31000 (Davidson)
pAra promoter reverse – BBa_B????? (Western)
RBS reverse – BBa_B????? (Western)
Davidson will complete the TT-RE assembly below and send to Western.
The Davidson cohort will also construct SuperParts necessary for the 1-pancake constructs in which the Kan resistance coding region is both forward and backward. Note: Davidson will need to send the forward KanR to Western for use in their assembly.
Upon receipt of the Hin Expression Unit (no Hix) from Western, Davidson will assemble the following and complete the assembly of Construct 2 and Construct 2 – Control.
