Construction Intermediates
From 2006.igem.org
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'''pBad Promoter- RBS''' BBa_J3102 [http://partsregistry.org/Part:BBa_J3102] | '''pBad Promoter- RBS''' BBa_J3102 [http://partsregistry.org/Part:BBa_J3102] | ||
+ | * Used culture #3 | ||
'''pBad Promoter-Hix''' : BBa_S03529 [http://partsregistry.org/Part:BBa_S03529] | '''pBad Promoter-Hix''' : BBa_S03529 [http://partsregistry.org/Part:BBa_S03529] | ||
+ | *Used the Midi-prepped DNA that we recieved from the Mammoths | ||
+ | |||
+ | '''pLac Promoter-RBS''' : | ||
'''pBad Promotor-Hix-RBS''' : BBa_S03530 [http://partsregistry.org/Part:BBa_S03530] | '''pBad Promotor-Hix-RBS''' : BBa_S03530 [http://partsregistry.org/Part:BBa_S03530] | ||
+ | * Used culture #3 | ||
'''Recombinational Enhancer- Double Forward terminator'''- BBa_S03512 [http://partsregistry.org/Part:BBa_S03512] | '''Recombinational Enhancer- Double Forward terminator'''- BBa_S03512 [http://partsregistry.org/Part:BBa_S03512] | ||
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'''Double Forward Terminator- Recombinational Enhancer''': BBa_S03516 [http://partsregistry.org/Part:BBa_S03516] | '''Double Forward Terminator- Recombinational Enhancer''': BBa_S03516 [http://partsregistry.org/Part:BBa_S03516] | ||
+ | *Used culture #1 | ||
'''HinLVA-Double Forward Terminator''': BBa_S03514 [http://partsregistry.org/Part:BBa_S03514] | '''HinLVA-Double Forward Terminator''': BBa_S03514 [http://partsregistry.org/Part:BBa_S03514] | ||
+ | * Used culture #1 | ||
'''Hin-Double Forward Terminator''': BBa_S03515 [http://partsregistry.org/Part:BBa_S03515] | '''Hin-Double Forward Terminator''': BBa_S03515 [http://partsregistry.org/Part:BBa_S03515] | ||
+ | * Used culutre #3 | ||
'''Hix-Double Forward Terminator-RE''' : BBa_S03522 [http://partsregistry.org/Part:BBa_S03522] | '''Hix-Double Forward Terminator-RE''' : BBa_S03522 [http://partsregistry.org/Part:BBa_S03522] | ||
+ | *Used culture #1 | ||
'''TetR Forwards-HixC''': BBa_S03523 [http://partsregistry.org/Part:BBa_S03523] | '''TetR Forwards-HixC''': BBa_S03523 [http://partsregistry.org/Part:BBa_S03523] | ||
+ | * Used culutre #2 | ||
'''TetR Forwards-Hix- Double Forward Terminator-RE''': BBa_S03524 [http://partsregistry.org/Part:BBa_S03524] | '''TetR Forwards-Hix- Double Forward Terminator-RE''': BBa_S03524 [http://partsregistry.org/Part:BBa_S03524] | ||
+ | * Used culture #2 | ||
'''Hix-TetR Forwards-Hix-Double Forward Terminator-RE''': BBa_S03527 [http://partsregistry.org/Part:BBa_S03527] | '''Hix-TetR Forwards-Hix-Double Forward Terminator-RE''': BBa_S03527 [http://partsregistry.org/Part:BBa_S03527] | ||
+ | * Used culture # 2 | ||
'''TetR Backwards-Hix''' : BBa_S03525 [http://partsregistry.org/Part:BBa_S03525] | '''TetR Backwards-Hix''' : BBa_S03525 [http://partsregistry.org/Part:BBa_S03525] | ||
+ | * Used culture #4 | ||
'''TetR Backwards-Hix- Double Forward Terminator-RE''': BBa_S03526 [http://partsregistry.org/Part:BBa_S03526] | '''TetR Backwards-Hix- Double Forward Terminator-RE''': BBa_S03526 [http://partsregistry.org/Part:BBa_S03526] | ||
+ | * Used culture # 3 | ||
'''Hix-TetR Backwards-Hix-Double Forward Terminator-RE''': BBa_S03528 [http://partsregistry.org/Part:BBa_S03528] | '''Hix-TetR Backwards-Hix-Double Forward Terminator-RE''': BBa_S03528 [http://partsregistry.org/Part:BBa_S03528] | ||
+ | *Used culture #3 |
Revision as of 15:16, 2 August 2006
pBad Promoter- RBS BBa_J3102 [http://partsregistry.org/Part:BBa_J3102]
- Used culture #3
pBad Promoter-Hix : BBa_S03529 [http://partsregistry.org/Part:BBa_S03529]
- Used the Midi-prepped DNA that we recieved from the Mammoths
pLac Promoter-RBS :
pBad Promotor-Hix-RBS : BBa_S03530 [http://partsregistry.org/Part:BBa_S03530]
- Used culture #3
Recombinational Enhancer- Double Forward terminator- BBa_S03512 [http://partsregistry.org/Part:BBa_S03512]
- This part has a twin, S03513, that was made accidentally
- Both parts were created on the registry by accident. It was never made in the laboratory.
Double Forward Terminator- Recombinational Enhancer: BBa_S03516 [http://partsregistry.org/Part:BBa_S03516]
- Used culture #1
HinLVA-Double Forward Terminator: BBa_S03514 [http://partsregistry.org/Part:BBa_S03514]
- Used culture #1
Hin-Double Forward Terminator: BBa_S03515 [http://partsregistry.org/Part:BBa_S03515]
- Used culutre #3
Hix-Double Forward Terminator-RE : BBa_S03522 [http://partsregistry.org/Part:BBa_S03522]
- Used culture #1
TetR Forwards-HixC: BBa_S03523 [http://partsregistry.org/Part:BBa_S03523]
- Used culutre #2
TetR Forwards-Hix- Double Forward Terminator-RE: BBa_S03524 [http://partsregistry.org/Part:BBa_S03524]
- Used culture #2
Hix-TetR Forwards-Hix-Double Forward Terminator-RE: BBa_S03527 [http://partsregistry.org/Part:BBa_S03527]
- Used culture # 2
TetR Backwards-Hix : BBa_S03525 [http://partsregistry.org/Part:BBa_S03525]
- Used culture #4
TetR Backwards-Hix- Double Forward Terminator-RE: BBa_S03526 [http://partsregistry.org/Part:BBa_S03526]
- Used culture # 3
Hix-TetR Backwards-Hix-Double Forward Terminator-RE: BBa_S03528 [http://partsregistry.org/Part:BBa_S03528]
- Used culture #3