ETH 2006 Half adder
From 2006.igem.org
(Difference between revisions)
m (→Selected XOR/AND Model Variants) |
|||
| Line 20: | Line 20: | ||
**Bacteria does not express fluorescent protein when pattern on the plate and picture match (no light and no chemical) | **Bacteria does not express fluorescent protein when pattern on the plate and picture match (no light and no chemical) | ||
**Bacteria gets red when pattern on the plate and picture do not match | **Bacteria gets red when pattern on the plate and picture do not match | ||
| + | |||
| + | ==Implementation== | ||
====Implementation from the ingenieur point of view==== | ====Implementation from the ingenieur point of view==== | ||
| Line 32: | Line 34: | ||
*we would need to give a kind of first signal to the system (to don't have light reactions before a certain moment, otherwise we have to work in a dark room...), an activation signal after the bacterias have grown on the plate | *we would need to give a kind of first signal to the system (to don't have light reactions before a certain moment, otherwise we have to work in a dark room...), an activation signal after the bacterias have grown on the plate | ||
| - | ====Chemical==== | + | ====Chemical Sensing==== |
*Lactate lacI represses, IPTG induces (BBa_R0011 or BBa_R0010 ) | *Lactate lacI represses, IPTG induces (BBa_R0011 or BBa_R0010 ) | ||
*Tetracycline, TetR inhibitor, Tet inducer by inhibiting TetR (or aTc, it's analog) (BBa_R0040) | *Tetracycline, TetR inhibitor, Tet inducer by inhibiting TetR (or aTc, it's analog) (BBa_R0040) | ||
| Line 39: | Line 41: | ||
* I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. since the inducers are water-soluble we would have to fix the chemicals onto the petro dish. | * I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. since the inducers are water-soluble we would have to fix the chemicals onto the petro dish. | ||
| - | ==== | + | ==Parts== |
| + | add parts here... | ||
| + | |||
| + | ==Modelling== | ||
=====Selected XOR/AND Model Variants===== | =====Selected XOR/AND Model Variants===== | ||
* [[media:eth-parts-and-xor.pdf|eth-parts-and-xor.pdf]] (∼0.5M) [[media:eth-parts-and-xor2.ppt|eth-parts-and-xor.ppt]] (∼0.5M) | * [[media:eth-parts-and-xor.pdf|eth-parts-and-xor.pdf]] (∼0.5M) [[media:eth-parts-and-xor2.ppt|eth-parts-and-xor.ppt]] (∼0.5M) | ||
| Line 48: | Line 53: | ||
*[[Media:AND34.ppt|AND34.ppt]] | *[[Media:AND34.ppt|AND34.ppt]] | ||
| - | + | == Pro's & Con's== | |
''Pro's:'' | ''Pro's:'' | ||
* meaningful from engineering point | * meaningful from engineering point | ||
Revision as of 11:52, 15 August 2006
back to → ETH 2006 Main Page → Meat Monitor idea → other project ideas
Contents |
Half-adder or Full-adder
An implementation of a half-adder or full-adder: 1-bit adder with carry
- [http://en.wikipedia.org/wiki/Full_adder half/full adder in wikipedia]
Idea: Pattern recognition
→ Illustration of the concepts:
Main Goal:
- Write something with a chemical on a petri plate (like ETH for example)
- Let Bacteria grow uniformly on the plate
- Expose the plate to a picture (black and white) of the same pattern
- Result:
- Bacteria gets green when pattern on the plate and picture match (light and chemical)
- Bacteria does not express fluorescent protein when pattern on the plate and picture match (no light and no chemical)
- Bacteria gets red when pattern on the plate and picture do not match
Implementation
Implementation from the ingenieur point of view
- Half Adder
- = opportunity to build an AND and an XOR Gate
Implementation from the biologist point of view
- AND Gate: presence of chemical induces the production of an inactive protein, which is activated by a second protein (produced when light is present): the first protein is then activating GFP production
- XOR Gate: I think Marko had a good idea about that (I didn't really got it)
- a light sensible promoter (there are some, see [http://partsregistry.org/Featured_Parts:Light_Sensor here])
- a chemical sensible promoter (chemical to be defined)
- we would need to give a kind of first signal to the system (to don't have light reactions before a certain moment, otherwise we have to work in a dark room...), an activation signal after the bacterias have grown on the plate
Chemical Sensing
- Lactate lacI represses, IPTG induces (BBa_R0011 or BBa_R0010 )
- Tetracycline, TetR inhibitor, Tet inducer by inhibiting TetR (or aTc, it's analog) (BBa_R0040)
- combination thereof BBa_I13614 / 17 / 23 / 24 / 27 / 37 / 53)
- simple sugar Arabinose (BBa_R0080)
- I see the main difficulty in the spatial separation as the cells are growing in the petri dishes. since the inducers are water-soluble we would have to fix the chemicals onto the petro dish.
Parts
add parts here...
Modelling
Selected XOR/AND Model Variants
- eth-parts-and-xor.pdf (∼0.5M) eth-parts-and-xor.ppt (∼0.5M)
Old
- eth-xor.pdf (483KB)
- eth-and.pdf (185KB)
- AND34.ppt
Pro's & Con's
Pro's:
- meaningful from engineering point
- valuable parts for synthetic biology
- stepwise proceeding applicable (→ intermediate and partial results!)
- experiments visually attractive
- probably simple enough
Con's:
- cheap copy of “bio-film” project ([http://partsregistry.org/cgi/htdocs/SBC04/index.cgi iGEM 2004])
- sensational experiments, have little in common with half adder
- maybe that a big vision is lacking
- general/practical benefit? (counter-argument: is this required?)
- too simple?
