Boston University 2006:making antibiotic stocks
From 2006.igem.org
(Difference between revisions)
Revision as of 19:01, 7 September 2006
Use the following protocol to produce 5 1mL aliquots of stock solution of your desired antibiotic. Use a 1:1000 dilution to reach a working concentration from the stock (i.e. take 5uL from the Amp stock and add to 5mL of LB broth to produce 100ug/mL Amp + LB broth).
Materials
- Powder of given antibiotic
- dH20 and/or 100% ethanol
- 5mL Falcon tube(s)
- 5 1.5mL microcentrifuge tubes (per 5mL falcon tube)
Method
Table of concentrations goes here
- a
- b
Notes
- Store all the aliquots at -20C.
- Tet is light-sensative, so be sure to prevent exposure (i.e. wrap it in tinfoil or keep it in a dark box)
