Double Digest Guide
From 2006.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
5' | 5' | ||
--gca GAATTC GCGGCCGC T TCTAGA G --insert-- T ACTAGT A GCGGCCG CTGCAG gct-- | --gca GAATTC GCGGCCGC T TCTAGA G --insert-- T ACTAGT A GCGGCCG CTGCAG gct-- | ||
| + | |||
--cgt CTTAAG CGCCGGCG A AGATCT C ---------- A TGATCA T CGCCGGC GACGTC cga-- | --cgt CTTAAG CGCCGGCG A AGATCT C ---------- A TGATCA T CGCCGGC GACGTC cga-- | ||
</nowiki> | </nowiki> | ||
Revision as of 20:31, 13 September 2006
iGEM Double Digest Guide by Karmella Haynes, 2006
5' --gca GAATTC GCGGCCGC T TCTAGA G --insert-- T ACTAGT A GCGGCCG CTGCAG gct-- --cgt CTTAAG CGCCGGCG A AGATCT C ---------- A TGATCA T CGCCGGC GACGTC cga--
| Enzymes | Buffer | Temperature | Purpose |
