Construction Intermediates
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''' TetR Backwards- RBS Backwards-HixC- Double Forwards Terminator-RE'''<font color='blue'>(TB-RBS<sub>rev</sub>-H-TT-RE)</font color> BBa_S03534 [http://partsregistry.org/Part:BBa_S03534] | ''' TetR Backwards- RBS Backwards-HixC- Double Forwards Terminator-RE'''<font color='blue'>(TB-RBS<sub>rev</sub>-H-TT-RE)</font color> BBa_S03534 [http://partsregistry.org/Part:BBa_S03534] | ||
* We chose not to use this part for ligation. | * We chose not to use this part for ligation. | ||
| + | |||
| + | '''Double Terminator Forwards-RBS-TetR Forwards'''<font color='blue'>(TT<sub>fwd</sub>-RBS-TF)</font color> BBa_S03595 [http://partsregistry.org/Part:BBa_S03595] | ||
| + | * Constructed in plasmid pSB1AK3, then put into plasmid pSB1A2. Used culture #'s 1, 2 and 3 (combined) | ||
Latest revision as of 21:41, 14 September 2006
Nicknames for the parts are in blue
pBad Promoter- RBS BBa_J3102 [http://partsregistry.org/Part:BBa_J3102]
- Used culture #3
pBad Promoter-Hix : BBa_S03529 [http://partsregistry.org/Part:BBa_S03529]
- Used the Midi-prepped DNA that we recieved from the Mammoths
pLac Promoter-RBS : BBa_S03511 [http://partsregistry.org/Part:BBa_S03511]
- Used culture #4
pBad Promotor-Hix-RBS (P-H-R) : BBa_S03530 [http://partsregistry.org/Part:BBa_S03530]
- Used culture #3
Recombinational Enhancer- Double Forward terminator(RE-TT): BBa_S03512 [http://partsregistry.org/Part:BBa_S03512]
- This part has a twin, S03513, that was made accidentally
- Both parts were created on the registry by accident. It was never made in the laboratory.
Double Forward Terminator- Recombinational Enhancer (TT-RE) : BBa_S03516 [http://partsregistry.org/Part:BBa_S03516]
- Used culture #1
HinLVA-Double Forward Terminator(HLVA-TT): BBa_S03514 [http://partsregistry.org/Part:BBa_S03514]
- Used culture #1
Hin-Double Forward Terminator(H-TT): BBa_S03515 [http://partsregistry.org/Part:BBa_S03515]
- Used culutre #3
Hix-Double Forward Terminator-RE(H-TT-RE) : BBa_S03522 [http://partsregistry.org/Part:BBa_S03522]
- Used culture #1
TetR Forwards-HixC(TF-H): BBa_S03523 [http://partsregistry.org/Part:BBa_S03523]
- Used culutre #2
TetR Forwards-Hix- Double Forward Terminator-RE(TF-H-TT-RE): BBa_S03524 [http://partsregistry.org/Part:BBa_S03524]
- Used culture #2
Hix-TetR Forwards-Hix-Double Forward Terminator-RE(H-TF-H-TT-RE) : BBa_S03527 [http://partsregistry.org/Part:BBa_S03527]
- Used culture # 2
TetR Backwards-Hix(TB-H) : BBa_S03525 [http://partsregistry.org/Part:BBa_S03525]
- Used culture #4
TetR Backwards-Hix- Double Forward Terminator-RE(TB-H-TT-RE) : BBa_S03526 [http://partsregistry.org/Part:BBa_S03526]
- Used culture # 3
Hix-TetR Backwards-Hix-Double Forward Terminator-RE(H-TB-H-TT-RE): BBa_S03528 [http://partsregistry.org/Part:BBa_S03528]
- Used culture #3
TetR Backwards-RBS(backwards)(TB-RBSrev) : BBa_S03532 [http://partsregistry.org/Part:BBa_S03532]
- Used culture #2
pBad-Hix C- TetR Backwards-RBS (backwards)(P-H-TB-RBSrev) : BBa_S03533 [http://partsregistry.org/Part:BBa_S03533]
- Used culture #1
TetR Backwards- RBS Backwards-HixC- Double Forwards Terminator-RE(TB-RBSrev-H-TT-RE) BBa_S03534 [http://partsregistry.org/Part:BBa_S03534]
- We chose not to use this part for ligation.
Double Terminator Forwards-RBS-TetR Forwards(TTfwd-RBS-TF) BBa_S03595 [http://partsregistry.org/Part:BBa_S03595]
- Constructed in plasmid pSB1AK3, then put into plasmid pSB1A2. Used culture #'s 1, 2 and 3 (combined)
