Presentation Outline
From 2006.igem.org
(Difference between revisions)
Macampbell (Talk | contribs) |
Macampbell (Talk | contribs) |
||
| Line 15: | Line 15: | ||
** Mathematics behind solution and possible number of flips per n pancakes | ** Mathematics behind solution and possible number of flips per n pancakes | ||
** Describe Biological equivalent problem | ** Describe Biological equivalent problem | ||
| - | + | ---- | |
'''Bubble Topics - split as time demands''' | '''Bubble Topics - split as time demands''' | ||
* ''Methods'' | * ''Methods'' | ||
| Line 24: | Line 24: | ||
** Using modeling to choose which families of pancake stacks to build | ** Using modeling to choose which families of pancake stacks to build | ||
** Distinguishing 1,2 from -2,1 → add in promoterless RFP | ** Distinguishing 1,2 from -2,1 → add in promoterless RFP | ||
| - | + | ---- | |
'''Davidson Presentation''' | '''Davidson Presentation''' | ||
* Data | * Data | ||
Revision as of 18:59, 21 September 2006
Western Presentation
- Define our goals
- Solve a mathematical problem using bacteria
- Integrate math and biology students holistically
- Work in tandem with two campuses to test parallel processing of PUIs for iGEM
- Design a device that would be more than just a widget
- Have a lot of fun learning
- Introduction
- Synthetic biology
- tests our understanding of biological units and
- allows us to design new devices using DNA.
- Define burnt pancake problem
- Schematic design of 1, 2, 3, and 4 pancake stacks
- Mathematics behind solution and possible number of flips per n pancakes
- Describe Biological equivalent problem
- Synthetic biology
Bubble Topics - split as time demands
- Methods
- Hin (+/- LVA)and Hix and RE and Fis (list of basic parts)
- How to assemble small DNA segments too big for oligos (Lance’s web site)
- How to generate backwards biobrick parts with PCR (switch-a-roo)
- Modeling the behavior of pancake flipping – deducing kinetics and size biases
- Using modeling to choose which families of pancake stacks to build
- Distinguishing 1,2 from -2,1 → add in promoterless RFP
Davidson Presentation
- Data
- Promoterless RBS+TF = tet resistant
- Backwarks Tet + backwards RBS = tet resistant
- Therefore, pSB1A2 and 3 have readthrough transcription in both directions
- Design and build pSB1A4 (describe construction and demonstrate function)
- Combine Hix with 1 pancake stacks (promoter-flavored and coding-flavored)
- Uncontrolled flipping
- Western blot of Hin….
- Building 2 pancakes….
- Consequences of Devices:
- Data storage
- Improved transgenic organisms (two states)
- Proof-of-concept for bacterial computers
- Next steps
- Conclusions
- Can solve problem but need control over kinetics
- Math and Biology meshed really well – even uncovered a new proof
- Multiple campuses can increase capacity through communication and cooperation. Size of school is not a limiting factor.
- First in vivo controlled flipping of DNA??
- We had a blast and learned heaps.
