Swimmy Bacteria : Chiba 2006
From 2006.igem.org
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*'''A.Bren and M.Eisenbach''' (2000) -- How Signals Are Heard during Bacterial Chemotaxis: Protein-Protein Interactions in Sensory Signal Propagation (J.Bacteriol.) | *'''A.Bren and M.Eisenbach''' (2000) -- How Signals Are Heard during Bacterial Chemotaxis: Protein-Protein Interactions in Sensory Signal Propagation (J.Bacteriol.) | ||
*'''P.Ames and J.S.Parkinson''' (1988) -- Transmembrane Signaling by Bacterial Chemoreceptors: E.coli Transducers with Locked Signal Output (Cell) | *'''P.Ames and J.S.Parkinson''' (1988) -- Transmembrane Signaling by Bacterial Chemoreceptors: E.coli Transducers with Locked Signal Output (Cell) | ||
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| - | Experiments | + | ==Experiments== |
| + | *''Chemotaxis Assay'' -- '''J.Adler''' -- A Method for Measuring Chemotaxis and Use of the Method to Determine Optimum Conditions for Chemotaxis by Escherichia coli | ||
*'''M.K.Slocum and J.S.Parkinson''' (1985) -- Genetics of Methyl-Accepting Chemotaxis Proteins in Escherichia coli: Null Phenotypes of the tar and tap Genes (J.Bacteriol.) | *'''M.K.Slocum and J.S.Parkinson''' (1985) -- Genetics of Methyl-Accepting Chemotaxis Proteins in Escherichia coli: Null Phenotypes of the tar and tap Genes (J.Bacteriol.) | ||
*'''J.S.Parkinson''' (1976) --- cheA, cheB, and cheC of Escherichia coli and Their Role in Chemotaxis (J.Bacteriol.) | *'''J.S.Parkinson''' (1976) --- cheA, cheB, and cheC of Escherichia coli and Their Role in Chemotaxis (J.Bacteriol.) | ||
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=Last Update= | =Last Update= | ||
Revision as of 14:55, 14 October 2006
Contents |
Concept
Swimmy Bacteria -- the E.coli freeze and gather when they are caught by red light.
red light on -> stop
red light off -> move
Control the E.coli's movement with light!
How it works
Members
Chiba 2006 team あ
Experiments
- 10/11 we're finding the suitable media to observe the E.coli's movement(chemotaxis). also we're doing the light cannon.
- 8/23 we made the tsr gene parts by PCR & discussed about the locked Tsr mutants.
Todo List
Referenses
Locked Tsr Mutants
- A.Bren and M.Eisenbach (2000) -- How Signals Are Heard during Bacterial Chemotaxis: Protein-Protein Interactions in Sensory Signal Propagation (J.Bacteriol.)
- P.Ames and J.S.Parkinson (1988) -- Transmembrane Signaling by Bacterial Chemoreceptors: E.coli Transducers with Locked Signal Output (Cell)
- P.Ames and J.S.Parkinson (1994) -- Constitutively Signaling Fragments of Tsr, the Escherichia coli Serine Chemoreceptor (J.Bacteriol.)
Experiments
- Chemotaxis Assay -- J.Adler -- A Method for Measuring Chemotaxis and Use of the Method to Determine Optimum Conditions for Chemotaxis by Escherichia coli
- M.K.Slocum and J.S.Parkinson (1985) -- Genetics of Methyl-Accepting Chemotaxis Proteins in Escherichia coli: Null Phenotypes of the tar and tap Genes (J.Bacteriol.)
- J.S.Parkinson (1976) --- cheA, cheB, and cheC of Escherichia coli and Their Role in Chemotaxis (J.Bacteriol.)
Last Update
- 10/11 swimmy & gene pathway
- 8/23 refrences
- 8/7 concept&members --maiko
