Testing Results
From 2006.igem.org
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<li>Module 1 (DH5a-z1): IPTG Induction of GFP | <li>Module 1 (DH5a-z1): IPTG Induction of GFP | ||
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<li>Absorbance of Arabinose, and Abs/Emi Spectra of GFP/mRFP | <li>Absorbance of Arabinose, and Abs/Emi Spectra of GFP/mRFP | ||
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Revision as of 03:10, 16 October 2006
Overview
We decided to integrate check-points into our construction plan, in order to test intermediary part functionality.
There are three basic modules:
- Module 1: Arabinose inducible pBad/araC promoter (I0500) produces temperature-sensitive LacI (LacI ts), which then, upon dimerization, represses the IPTG inducible LacI+ pL promoter (R0011) resulting in a decrease in GFP (E0240)/mRFP (I13507)
- Module 2: In Module 1, insert DNA sequence coding for 434 cI protein instead of the reporters (GFP/mRFP) and then add Prm+ promoter (I12006), which is repressed by 434 cI and add GFP
- Module 3: Take Module 3 and add mRFP (J04450)
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Completed Tests
- Module 1 (DH5a): IPTG Induction of mRFP
- Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP
- Module 1 (DH5a-z1): IPTG Induction of GFP
- Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP
- Module 1 (DH5a-z1): Fluorescnece Images of GFP/mRFP
- Module 1 (DH5a-z1): IPTG Induction of GFP/mRFP
- Module 1 (DH5a-z1): IPTG Induction of GFP
back to University_of_Toronto_2006
Future Tests
- Surface response (GFP/mRFP) to varying IPTG and Arabinose
- Absorbance of Arabinose, and Abs/Emi Spectra of GFP/mRFP
back to University_of_Toronto_2006
