Phase 1: PCR Amplification of Genes
From 2006.igem.org
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'''A. Isolating genes for the quorum sensing mechanism from genomic ''B. subtilis'' DNA''' | '''A. Isolating genes for the quorum sensing mechanism from genomic ''B. subtilis'' DNA''' | ||
| - | + | #ComX - pheromone, 168bp | |
| - | + | ::Primers: Fwd = CGGAATTCGCTCTAGATGCAAGACCTAATTAACTACTTTTTAAATTATCC | |
Rev = GACTGCAGCTACTAGTTAATCACCCCATTGACGGGTTATTGG | Rev = GACTGCAGCTACTAGTTAATCACCCCATTGACGGGTTATTGG | ||
| - | + | :::Forward primer contains EcoRI and XbaI cut sites; Reverse primer contains SpeI and PstI cut sites | |
| - | + | :::Amplification of ComX alone was unsuccessful. However, ComQ and ComX are adjacent on the genome so we subsequently amplified them together | |
| - | + | ||
Revision as of 20:52, 23 October 2006
A. Isolating genes for the quorum sensing mechanism from genomic B. subtilis DNA
- ComX - pheromone, 168bp
- Primers: Fwd = CGGAATTCGCTCTAGATGCAAGACCTAATTAACTACTTTTTAAATTATCC
Rev = GACTGCAGCTACTAGTTAATCACCCCATTGACGGGTTATTGG
- Forward primer contains EcoRI and XbaI cut sites; Reverse primer contains SpeI and PstI cut sites
- Amplification of ComX alone was unsuccessful. However, ComQ and ComX are adjacent on the genome so we subsequently amplified them together
