Projects
From 2006.igem.org
(Difference between revisions)
Revision as of 10:59, 28 October 2006
The projects
Regulation of Bacterial Chemotaxis
- Use two attractants whose receptors are inducible (serine with Tar, aspartate with Tsr). These define two orthogonal axes. Use a default attractant along a diagonal to establish '-' movement. Immediate goals: To establish substrate geometries upon which multiple reproducible, stable, quantitative chemical gradients can be established; to validate default chemotaxis in the absence of MCP receptors.
Synchronization of bacterial cell cycles
- Synchronization of bacterial cell cycles. Use a cell cycle-dependent promoter to drive a LuxI-LuxR based cell-cell signal. Use regulation of replication initiator DnaA to modulate cell cycle in receiver cells. Immediate goals: To determine if candidate promoters oscillate; to regulate DnaA levels.
Monitoring long-term effects of UV exposure
- Analysis of stress responses in biofilms. Connect stress response system to a lac based bistable GFP reporter. This allows transient expression at a stress-response promoter to trigger persistent expression of GFP. Immediate goals: To standardize biofilm assay; to design other experiments in which such a construct would be useful, especially using a cell-sorter.
