Swimmy Bacteria : Chiba 2006
From 2006.igem.org
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'''Control the E.coli's movement with light!''' | '''Control the E.coli's movement with light!''' | ||
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to stop the e.coli by light, we needed a part that stop the e.coli, and combine this with a light receptor that UCSF made it last year. | to stop the e.coli by light, we needed a part that stop the e.coli, and combine this with a light receptor that UCSF made it last year. | ||
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we chose to control chemotaxis. | we chose to control chemotaxis. | ||
We've found out, from the paper(..see refrences), the chemoreceptor that fix the e.coli tumble. | We've found out, from the paper(..see refrences), the chemoreceptor that fix the e.coli tumble. | ||
| - | it is a fragment of the chemoreceptor "Tsr" (a serin receptor) | + | it is a fragment of the chemoreceptor "Tsr" (a serin receptor)from aa 290 to 551. we call this tsr-cw(or tsr290). |
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<br /> | <br /> | ||
[[Image:pathway.jpg|pathway]] | [[Image:pathway.jpg|pathway]] | ||
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| + | =Experiments= | ||
| + | we made the new part rbs-tsrcw by PCR and made a new BioBrick (BBa_J29049). | ||
| + | |||
| + | ==Swarm Plate Assay== | ||
| + | first we wanted to make sure this parts will work, so we put it suffix in the OmpC-promotor& araC-promotor. | ||
| + | we found the OmpC promotor (R0082) was not working good, so then we used the araC promotor. | ||
| + | -- results under construction -- | ||
| + | |||
| + | |||
| + | ==Capillary Assay== | ||
| + | we wanted to see if the tsr-cw is working good with this assay,too. | ||
| + | we're waiting for the results... | ||
=Members= | =Members= | ||
Revision as of 16:09, 29 October 2006
Contents |
Concept
Swimmy Bacteria -- the E.coli freeze and gather when they are caught by red light.
red light on -> stop
red light off -> move
Control the E.coli's movement with light!
Design
to stop the e.coli by light, we needed a part that stop the e.coli, and combine this with a light receptor that UCSF made it last year.
there was some ways to stop the e.coli running:
- making a chemaera with chemotaxis receptor and light sensing receptor
- stop the motor protein's expression(motB protein: from last year's parts)
- controling chemotaxis
etc
we chose to control chemotaxis. We've found out, from the paper(..see refrences), the chemoreceptor that fix the e.coli tumble. it is a fragment of the chemoreceptor "Tsr" (a serin receptor)from aa 290 to 551. we call this tsr-cw(or tsr290).
Experiments
we made the new part rbs-tsrcw by PCR and made a new BioBrick (BBa_J29049).
Swarm Plate Assay
first we wanted to make sure this parts will work, so we put it suffix in the OmpC-promotor& araC-promotor. we found the OmpC promotor (R0082) was not working good, so then we used the araC promotor. -- results under construction --
Capillary Assay
we wanted to see if the tsr-cw is working good with this assay,too. we're waiting for the results...
Members
Chiba 2006 team あ
Experiments
light cannon
- 10/15 we wrote a picture at the solid media.(not finished yet) also we changed the arabinose concentration at the liquid media.
- 10/14 the Fluorescent lamp was the best light. we could see the on/off clearly in the solid media. we found that the thin media is better than the thicker one.
- 10/11 we changed the arabinose & S-gal's concentration & the light sorce to find the best condition.
swimmy
- 10/15 tried the capiraly assay to see chemotaxis. also we washed the e.coli and observed it with the microscope(but it was kind of hard)
- 10/11 we're finding the suitable media to observe the E.coli's movement(chemotaxis).
- 8/23 we made the tsr gene parts by PCR & discussed about the locked Tsr mutants.
Todo List
Referenses
Locked Tsr Mutants
- A.Bren and M.Eisenbach (2000) -- How Signals Are Heard during Bacterial Chemotaxis: Protein-Protein Interactions in Sensory Signal Propagation (J.Bacteriol.)
- P.Ames and J.S.Parkinson (1988) -- Transmembrane Signaling by Bacterial Chemoreceptors: E.coli Transducers with Locked Signal Output (Cell)
- P.Ames and J.S.Parkinson (1994) -- Constitutively Signaling Fragments of Tsr, the Escherichia coli Serine Chemoreceptor (J.Bacteriol.)
Experiments
- Microscope -- Real-time imaging of fluorescent flagellar filaments.J.bacteriol.2000
- Chemotaxis Assay -- J.Adler -- A Method for Measuring Chemotaxis and Use of the Method to Determine Optimum Conditions for Chemotaxis by Escherichia coli
- M.K.Slocum and J.S.Parkinson (1985) -- Genetics of Methyl-Accepting Chemotaxis Proteins in Escherichia coli: Null Phenotypes of the tar and tap Genes (J.Bacteriol.)
- J.S.Parkinson (1976) --- cheA, cheB, and cheC of Escherichia coli and Their Role in Chemotaxis (J.Bacteriol.)
Last Update
- 10/13 todo list
- 10/11 swimmy & gene pathway
- 8/23 refrences
- 8/7 concept&members --maiko
