Projects

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===The projects===
===The projects===
[[X-Y_chemotaxis|Regulation of Bacterial Chemotaxis]]
[[X-Y_chemotaxis|Regulation of Bacterial Chemotaxis]]
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* Use two attractants whose receptors are inducible (serine with Tar, aspartate with Tsr). These define two orthogonal axes. Use a default attractant along a diagonal to establish '-' movement. Immediate goals: To establish substrate geometries upon which multiple reproducible, stable, quantitative chemical gradients can be established; to validate default chemotaxis in the absence of MCP receptors.
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* Use two attractants whose receptors are inducible (serine with Tar, aspartate with Tsr). These define two orthogonal axes. Use a default attractant along a diagonal to establish '-' movement.  
[[Image:ncbs_igem2006_net1.jpg|thumb|none|160 px]] <br/>
[[Image:ncbs_igem2006_net1.jpg|thumb|none|160 px]] <br/>
[[Synchronization_of_Cell_Cycle|Synchronization of bacterial cell cycles]]
[[Synchronization_of_Cell_Cycle|Synchronization of bacterial cell cycles]]
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*Synchronization of bacterial cell cycles. Use a cell cycle-dependent promoter to drive a LuxI-LuxR based cell-cell signal. Use regulation of replication initiator DnaA to modulate cell cycle in receiver cells. Immediate goals: To determine if candidate promoters oscillate; to regulate DnaA levels.  
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*Synchronization of bacterial cell cycles. Use a cell cycle-dependent promoter to drive a LuxI-LuxR based cell-cell signal. Use regulation of replication initiator DnaA to modulate cell cycle in receiver cells.  
[[Image:ncbs_igem2006_net2.jpg|thumb|none|160 px]]
[[Image:ncbs_igem2006_net2.jpg|thumb|none|160 px]]
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[[Memory_effects_of_UV_exposure|Monitoring long-term effects of UV exposure]]
[[Memory_effects_of_UV_exposure|Monitoring long-term effects of UV exposure]]
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*Analysis of stress responses in biofilms. Connect stress response system to a lac based bistable GFP reporter. This allows transient expression at a stress-response promoter to trigger persistent expression of GFP. Immediate goals: To standardize biofilm assay; to design other experiments in which such a construct would be useful, especially using a cell-sorter.
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*Analysis of stress responses in biofilms. Connect stress response system to a lac based bistable GFP reporter. This allows transient expression at a stress-response promoter to trigger persistent expression of GFP.  
[[Image:ncbs_igem2006_net3.jpg|thumb|none|160 px]]
[[Image:ncbs_igem2006_net3.jpg|thumb|none|160 px]]
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Latest revision as of 04:08, 30 October 2006

IGEM Bangalore HOME

The projects

Regulation of Bacterial Chemotaxis

  • Use two attractants whose receptors are inducible (serine with Tar, aspartate with Tsr). These define two orthogonal axes. Use a default attractant along a diagonal to establish '-' movement.
Ncbs igem2006 net1.jpg

Synchronization of bacterial cell cycles

  • Synchronization of bacterial cell cycles. Use a cell cycle-dependent promoter to drive a LuxI-LuxR based cell-cell signal. Use regulation of replication initiator DnaA to modulate cell cycle in receiver cells.
Ncbs igem2006 net2.jpg


Monitoring long-term effects of UV exposure

  • Analysis of stress responses in biofilms. Connect stress response system to a lac based bistable GFP reporter. This allows transient expression at a stress-response promoter to trigger persistent expression of GFP.
Ncbs igem2006 net3.jpg



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