Projects
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===The projects=== | ===The projects=== | ||
[[X-Y_chemotaxis|Regulation of Bacterial Chemotaxis]] | [[X-Y_chemotaxis|Regulation of Bacterial Chemotaxis]] | ||
- | * Use two attractants whose receptors are inducible (serine with Tar, aspartate with Tsr). These define two orthogonal axes. Use a default attractant along a diagonal to establish '-' movement | + | * Use two attractants whose receptors are inducible (serine with Tar, aspartate with Tsr). These define two orthogonal axes. Use a default attractant along a diagonal to establish '-' movement. |
[[Image:ncbs_igem2006_net1.jpg|thumb|none|160 px]] <br/> | [[Image:ncbs_igem2006_net1.jpg|thumb|none|160 px]] <br/> | ||
[[Synchronization_of_Cell_Cycle|Synchronization of bacterial cell cycles]] | [[Synchronization_of_Cell_Cycle|Synchronization of bacterial cell cycles]] | ||
- | *Synchronization of bacterial cell cycles. Use a cell cycle-dependent promoter to drive a LuxI-LuxR based cell-cell signal. Use regulation of replication initiator DnaA to modulate cell cycle in receiver cells | + | *Synchronization of bacterial cell cycles. Use a cell cycle-dependent promoter to drive a LuxI-LuxR based cell-cell signal. Use regulation of replication initiator DnaA to modulate cell cycle in receiver cells. |
[[Image:ncbs_igem2006_net2.jpg|thumb|none|160 px]] | [[Image:ncbs_igem2006_net2.jpg|thumb|none|160 px]] | ||
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[[Memory_effects_of_UV_exposure|Monitoring long-term effects of UV exposure]] | [[Memory_effects_of_UV_exposure|Monitoring long-term effects of UV exposure]] | ||
- | *Analysis of stress responses in biofilms. Connect stress response system to a lac based bistable GFP reporter. This allows transient expression at a stress-response promoter to trigger persistent expression of GFP | + | *Analysis of stress responses in biofilms. Connect stress response system to a lac based bistable GFP reporter. This allows transient expression at a stress-response promoter to trigger persistent expression of GFP. |
[[Image:ncbs_igem2006_net3.jpg|thumb|none|160 px]] | [[Image:ncbs_igem2006_net3.jpg|thumb|none|160 px]] | ||
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<br style="clear:both" /> | <br style="clear:both" /> |
Latest revision as of 04:08, 30 October 2006
The projects
Regulation of Bacterial Chemotaxis
- Use two attractants whose receptors are inducible (serine with Tar, aspartate with Tsr). These define two orthogonal axes. Use a default attractant along a diagonal to establish '-' movement.
Synchronization of bacterial cell cycles
- Synchronization of bacterial cell cycles. Use a cell cycle-dependent promoter to drive a LuxI-LuxR based cell-cell signal. Use regulation of replication initiator DnaA to modulate cell cycle in receiver cells.
Monitoring long-term effects of UV exposure
- Analysis of stress responses in biofilms. Connect stress response system to a lac based bistable GFP reporter. This allows transient expression at a stress-response promoter to trigger persistent expression of GFP.