Balloon Bacteria : Chiba 2006
From 2006.igem.org
(→Results) |
|||
| Line 43: | Line 43: | ||
| - | == Experiments == | + | ==Experiments== |
#We made inducible rodA''mut''. | #We made inducible rodA''mut''. | ||
| Line 50: | Line 50: | ||
| - | == Results == | + | ==Results== |
'''1.rodA'mut'''' | '''1.rodA'mut'''' | ||
| Line 64: | Line 64: | ||
...show more.[[CI inverter]] | ...show more.[[CI inverter]] | ||
| - | == Member == | + | ==Member== |
[[Chiba University, Japan 2006|Chiba 2006]] | [[Chiba University, Japan 2006|Chiba 2006]] | ||
Revision as of 10:23, 30 October 2006
Contents |
Concept
The light in the morning leaking from a gap between the curtains... this morning lights calls and awakes the E. coli.
Suprise!!
The E. coli become bigger and bigger like BALLOON!!!
→World first bacteria that swells by lights.!!!
When growing...
The movement of the E. coli can be obvered with the naked eyes !
"Big" E. coli is easily sorted by FACS !
Make much more proteins/cell !
Design
First
The E. coli doesn't have photoreceptor. But...
We have Cph8(utwxas/uCSF;iGEM2005)in our hand
Second
The E. coli divides the cells by forming "partition". So...
'Expression of the enzyme in septem formation is knocked-down.
Third
E. coli rather "extend" due to the action of RodA. So...
We make inducible RodAmut.
Experiments
- We made inducible rodAmut.
- We made CI inverter and compared it.
Results
1.rodA'mut'
...show more.rodAmut
2.CI inverter
...show more.CI inverter
Member
Chiba 2006 http://chem.tf.chiba-u.jp/igem/ iGEM Chiba
『je』team
